Medium to promote islet cell survival
AssigneeMcGill University
InventorsLawrence Rosenberg, Dusica Maysinger
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Abstract
The present invention relates to a medium for promoting the survival of islet cells, which comprises one or more growth factors in combination with FK506 in amounts having an anti-apoptotic effect on islet cells in a physiologically acceptable culture medium.
Claims27
A medium for promoting the survival of islet cells, which comprises one or more growth factors in combination with FK506 in amounts having an anti-apoptotic effect on islet cells in a physiologically acceptable culture medium.
A method for producing a medium promoting islet cell survival, which comprises:
- a) preparing a tertiary pancreatic duct culture from primary duct epithelial islets;
- and
- b) purifying the prepared tertiary pancreatic duct culture and incubating the purified culture in a physiologically suitable medium containing one or more growth factors in combination with FK506 in an amount effective for maintaining viability of said islet cells when in contact therewith.
A medium for promoting the survival of islet cells comprising, in a physiologically culture medium, IGF-I or IGF-II in combination with NGF in an amount having a synergistic anti-apoptotic effect on islet cells.
Dependencies are inferred from the claim text.