System for directed molecular interaction in surface plasmon resonance analysis

A sample delivery and sensing unit for directed molecular interaction during surface plasmon resonance analysis comprising:

• an integrally formed surface plasmon resonance sensor having, in fixed disposition thereto, a housing transparent to a given frequency of light, a source of the given frequency of light, a mirror and a photodetector array;
• a surface plasmon resonance layer in optic communication with the integrally formed surface plasmon resonance sensor;
• and
• a flow cell adapted for attachment to the surface plasmon resonance layer, having a fluid path, an analyte detection chamber disposed along the fluid path and having an interior surface in fluidic communication with the surface plasmon resonance layer, and adapted for generation of a molecular interaction bias across the chamber.

A sample delivery and sensing unit for directed molecular interaction during surface plasmon resonance analysis comprising:

• an integrally formed surface plasmon sensor having, in fixed functional geometric alignment thereto, a housing transparent to electromagnetic radiation of a given frequency range, a source of electromagnetic radiation having the given frequency range, a photodetector array disposed adjacent the surface of the housing and substantially coplanar with the source, such that radiation from the source reflects off the surface and strikes the photodetector array;
• a thin surface plasmon resonance layer in optic communication with an exterior surface of the integrally formed surface plasmon resonance sensor;
• and
• an analyte detection chamber in fluidic communication with the surface plasmon resonance layer, adapted to generate a molecular interaction bias across the analyte detection chamber to direct bias responsive conjugated molecules to the surface plasmon resonance layer.

A method for kinetically controlled surface plasmon resonance analysis comprising:

• providing a surface plasmon resonance sensor having a surface plasmon layer in optical communication with the sensor;
• derivatizing the surface plasmon layer;
• placing an analyte detection chamber in fluidic communication with the derivatized surface plasmon layer, wherein the chamber is adapted to generate a molecular interaction bias across the chamber;
• providing a conjugate between an analyte and a bias responsive moiety, wherein the analyte is reactive with the derivatized surface plasmon layer and the bias responsive moiety changes the response of the analyte to the molecular interaction bias;
• introducing the conjugated analyte into the chamber;
• generating the molecular interaction bias;
• and
• determining changes in surface plasmon resonance due to association of the conjugated analyte to the derivatized surface plasmon layer.