Enhancing surface-generated fluorescence signal emitted by a sample
AssigneesCommissariat a l'Energie Atomique, Biomerieux
InventorsFrancois Perraut, Patrick Chaton, Patrick Pouteau
Life of this patent
Estimating the term…
Abstract
The invention relates to the amplification of the fluorescence emitted by an areal sample. The device (10) includes a support (11) transmitting all or part of the fluorescence signal and intended to support the areal sample (13), a thin layer (12) being interposed between the support (11) and the areal sample (13), the thin layer having a refractive index greater than the refractive index of the support and than the refractive index of the medium flooding the areal sample during a fluorescence measurement, the thickness of the thin layer being chosen so that the thin layer (12) transmits all or part of the fluorescence signal which is measured after passing through the support (12).
Claims10
A process for amplifying a fluorescence signal emitted by an areal sample (13) supported by a support (11) in response to an excitation signal, the support (11) transmitting all or part of the fluorescence signal, consisting in interposing a thin layer (12) between the support (11) and the areal sample (13), characterised in that the thin layer has a refractive index greater than the refractive index of the support and than the refractive index of the medium (30, 62) flooding the areal sample (13), the thickness of the thin layer being selected in such a way that the thin layer (12) transmits all or part of the fluorescence signal which is measured after passing through the support (11).
Dependencies are inferred from the claim text.