Skin substitutes with improved barrier function
AssigneeStratech Corporation
InventorsAllen Comer, Lynn Allen-Hoffmann, Michael Hoffmann
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Abstract
The present invention relates to in vitro cultured skin substitutes, and in particular to in vitro cultured skin substitutes that have improved barrier function. In some embodiments, improved barrier function is a result of improved culture conditions, while in other embodiments, improved barrier function results from genetic modification of keratinocytes. Improved culture conditions to improve barrier function include organotypic culture in the presence of linoleic acid and/or linoleic acid at about 75% humidity. Suitable genetic modifications for improving barrier function includes transfection with a DNA construct capable of expressing GKLF.
Claims54
A composition comprising a human skin equivalent, said skin equivalent having a surface electrical capacitance of from about 40 to about 240 pF.
Isolated keratinocytes comprising a DNA construct comprising a sequence encoding GKLF operably linked to an exogenous promoter.
A method of making skin equivalents having improved barrier function comprising:
- a) providing keratinocytes and a culture media comprising ascorbic acid and linoleic acid;
- b) culturing said keratinocytes under conditions such that a skin equivalent having improved barrier function is formed.
A method of making skin equivalents having improved barrier function comprising:
- a) providing keratinocytes and a DNA construct comprising a sequence encoding GKLF operably linked to an exogenous promoter;
- b) transfecting said keratinocytes with said DNA construct to provide transfected keratinocytes;
- and
- c) culturing said transfected keratinocytes under conditions such that a skin equivalent having improved barrier function is formed.
A method of screening compounds comprising
- a) providing a skin equivalent having a surface electrical capacitance of from about 40 to about 240 pF,
- b) treating said skin equivalent with said compound.
A kit comprising at least one skin equivalent having a surface electrical capacitance of from about 40 to about 240 pF.
Dependencies are inferred from the claim text.