Sustained totipotent regenerable tissue culture of Arundo donax (Giant Reed) and totipotent tissue and plants produced therefrom
OwnerLaszlo Marton, Mihaly Czakoinventors
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Abstract
A method is provided for generating sustained totipotent tissue cultures of giant reed (Arundo donax L.), and for micropropagating giant reed in vitro, wherein immature inflorescence are cultivated to produce totipotent tissue which is suitable for sustained maintenance and propagation. Greening of the tissue can be induced under light and the multishoot culture can multiply by microtillering. Foreign genes can be introduced into the tissue if desired, and the transgenic plants can be used in phytoremediation technologies in the field and in phytoreactors independently of seasons.
Claims31
A method for the production of totipotent tissue culture of giant reed (Arundo donax L.), the method comprising:
- selecting an explant of living tissue from Arundo donax L.;
- and
- cultivating the A. donax tissue on a primary medium to produce totipotent A. donax tissue.
A method for the micropropagation of giant reed (Arundo donax L.), the method comprising:
- selecting an explant of living tissue from Arundo donax L.;
- cultivating the A. donax tissue on a primary medium to produce a totipotent tissue culture;
- cultivating the totipotent A. donax totipotent tissue on a secondary medium to produce complete plantlets having roots and shoots;
- and
- acclimating the plantlets in soil.
A method for removal of an environmental pollutant from wastewater, the method comprising:
- providing at least 10 A. donax plants that possess the same genetic characteristics;
- establishing the plants in a liquid medium;
- and
- contacting the roots of the plants in the liquid medium with an environmental pollutant,
- thereby causing the environmental pollutant to be removed from the liquid medium.
A method for bioremediation of an environmental pollutant from a land area, the method comprising:
- providing at least 10 A. donax plants that possess the same genetic characteristics;
- establishing the plants in soil;
- and contacting the roots of the plants with the environmental pollutant in the land area,
- thereby causing the environmental pollutant to be removed from the land area.
Dependencies are inferred from the claim text.