Maize PR1 polynucleotides and methods of use
AssigneePioneer Hi-Bred International, Inc.
InventorsCarl R. Simmons, Pedro A. Navarro Acevedo, Virginia C. Crane
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Abstract
The present invention provides compositions and methods for enhancing the disease resistance of plants. The compositions are novel nucleic acid molecules isolated from maize, which encode pathogenesis-related (PR1) proteins, and the PR1 proteins encoded thereby. The methods comprise introducing into a plant cell at least one nucleotide construct comprising a PR1 nucleotide sequence of the invention operably linked to a promoter that drives expression in a plant cell. The methods additionally involve regenerating a stably transformed plant from the transformed plant cell. Transformed plants and seeds having enhanced disease resistance are also provided.
Claims20
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 3, 5, 7, 9, or 11;
- b) the nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 2, 4, 6, 8, 10, or 12;
- c) a nucleotide sequence having at least 80% identity to the coding region of at least one sequence set forth in a), wherein said nucleotide sequence is capable of enhancing the disease resistance of a plant when expressed in the plant;
- d) a nucleotide sequence that hybridizes under stringent conditions to at least one sequence that is complementary to a sequence set forth in a), wherein said nucleotide sequence is capable of enhancing the disease resistance of a plant when expressed in the plant;
- and
- e) a nucleotide sequence that is complementary to a sequence of a), b), c), or d).
A transformed plant comprising in its genome at least one stably incorporated nucleotide construct comprising a PR1 nucleotide sequence operably linked to a promoter that drives expression of a coding sequence in a plant cell, wherein said PR1 nucleotide sequence is selected from the group consisting of:
- a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 3, 5, 7, 9, or 11;
- b) the nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 2, 4, 6, 8, 10, or 12;
- c) a nucleotide sequence having at least 80% identity to the coding region of at least one sequence set forth in a), wherein said nucleotide sequence is capable of enhancing the disease resistance of a plant when expressed therein;
- d) a nucleotide sequence that hybridizes under stringent conditions to at least one sequence that is complementary to a sequence set forth in a), wherein said nucleotide sequence is capable of enhancing the disease resistance of a plant when expressed therein;
- and
- e) a nucleotide sequence that is complementary to a sequence of a), b), c), or d).
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
- a) the amino acid sequence set forth in SEQ ID NO:
- 2, 4, 6, 8, 10, or 12;
- b) an amino acid sequence having at least 80% identity to at least one sequence set forth in a), wherein said polypeptide is capable of enhancing the disease resistance of a plant;
- and
- c) the amino acid sequence encoded by the nucleotide sequence set forth in SEQ ID NO:
- 1, 3, 5, 7, 9, or 11.
Dependencies are inferred from the claim text.