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US 2002/0166141 A1Published application
Antimicrobial peptides and methods of use
AssigneePioneer Hi-Bred International, Inc.
InventorsCarl R. Simmons, Pedro A. Navarro Acevedo
Life of this patent
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Abstract
The invention provides isolated KCP-like nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering KCP-like nucleic acid and/or protein concentration and/or composition of plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.
Claims16
An isolated nucleic acid molecule comprising a polynucleotide sclected from the group consisting of:
- a. a polynucleotide that encodes a polypeptide of SEQ ID NOS:37-72;
- b. a polynucleotide comprising at least 20 contiguous bases of SEQ ID OS:1-36;
- c. a polynucleotide having at least 70% sequence identity to any of SEQ ID NOS:1-36, wherein said polynucleotide encodes a polypeptide having KCP-like activity;
- d. a polynucleotide at least 25 nucleotides in length that hybridizes to a polynucleotide having the sequence set forth in SEQ ID NOS:1-36, wherein said polynucleotide encodes a polypeptide having KCP-like activity;
- e. a polynucleotide comprising the sequence set forth in any of SEQ ID NOS:1-36;
- and,
- f. a polynucleotide complementary to a polynucleotide of (a) through (e).
4 more elements
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
- a. an amino acid sequence comprising at least 25 contiguous amino acids of the sequence set forth in SEQ ID NOS:37-72;
- b. an amino acid sequence having at least 75% sequence identity to the sequence set forth in SEQ ID NOS:37-72, wherein said polypeptide retains KCP-like activity;
- and,
- c. an amino acid sequence comprising the sequences set forth in SEQ ID NOS:37-72.
1 more element
A method for identifying KCP-like proteins, said method comprising:
- a. searching at least one protein database with a pattern selected from the group consisting of:
- i) a pattern representing a compound having the formula (SEQ ID NO:97)
- C-X(2)-C-C-X(2)-[CS]-X(1,2)-C-V-P-[PSATK]-[GR]-X(2)-[GAQR], wherein:
- C is cysteine;
- X(2) is any two amino acids selected independently from one another;
- [CS] is one amino acid selected from the group consisting of cysteine and serine;
- X(1,2) is X(1) or X(2) wherein X(1) is any one amino acid, and
- X(2) is any two amino acids selected independently from one another;
- V is valine;
- P is proline;
- [PSATK] is one amino acid selected from the group consisting of proline, serine, alanine, threonine, and lysine;
- [GR] is one amino acid selected from the group consisting of glycine and arginine;
- and
- [GAQR] is one amino acid selected from the group consisting of glycine, alanine, glutamine and arginine;
- and
- ii) a pattern for a compound having the formula (SEQ ID NO:98)
- [CS]-[PSQAG]-X(0,2)-C-Y-X(4)-[TNSM]-X(5,8)-K, wherein
- [CS] is one amino acid selected from the group consisting of cysteine and serine;
- [PSQAG] is one amino acid selected from the group consisting of proline, serine, glutamine, alanine, and glycine;
- X(0,2) is X(0) or X(1) or X(2) wherein X(0) is no amino acid, X(1) is any one amino acid, and X(2) is any two amino acids selected independently from one another;
- C is cysteine;
- Y is tyrosine;
- X(4) is any four amino acids selected independently froni one another;
- [TNSM] is one amino acid selected from the group consisting of threonine, asparagine, serine, and methionine;
- X(5,8) is X(5) or X(6) or X(7) or X(8) wherein X(5) is any five amino acids selected independently from one another, X(6) is any six amino acids selected independently from one another, X(7) is any seven amino acids selected independently from one another, and X(8) is any eight amino acids selected independently from one another;
- and
- K is lysine;
- and
- b. selecting among retrieved proteins at least one protein comprising at least one amino acid sequence represented by at least one formula selected from said group.
26 more elements
Dependencies are inferred from the claim text.