Mutated barnase gene and plant transformed by the same
OwnerKazuyuki Hamada, Fumio Nakakidoinventors
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Abstract
A male sterile plant free from any undesirable characteristic is constructed by anther-specifically expressing barnase gene alone. A barnase gene, which has a mutation and thus sustains its activity at a lowered level, is transferred into a plant and anther-specifically expressed, thereby efficiently providing a male sterile transgenic plant.
Claims10
A mutant barnase gene having one or some mutations at least in part of the DNA sequence of barnase gene, wherein said mutant barnase gene is capable, when anther-specifically expressed in a plant, of making said plant substantially male sterile without exerting any substantially disadvantageous effect on the tissues except for the anthers.
A gene which is derived from a DNA sequence encoding the same amino acid sequence as shown in SEQ ID NO:
- 1 by substitution, deletion, insertion or addition of one to several nucleotides in said DNA sequence, wherein said gene is capable of encoding a protein which makes a plant substantially male sterile when expressed anther-specifically in said plant.
A gene comprising a DNA sequence which is derived from a DNA sequence encoding the same amino acid sequence as shown in SEQ ID NO:
- 1 by substitution, deletion, insertion or addition of one to several nucleotides in the latter DNA sequence, and a promoter located upstream of said DNA sequence for allowing an anther-specific expression, wherein said gene is capable of making a plant substantially male sterile when introduced into the genome of said plant.
A gene represented by SEQ ID NO:
- 3 which encodes a protein capable of making a plant substantially male sterile when expressed anther-specifically in said plant.
A gene comprising the sequence represented by SEQ ID NO:
- 3 and a promoter located upstream of said sequence for allowing an anther-specific expression, wherein said gene is capable of making a plant substantially male sterile when introduced into the genome of said plant.
Dependencies are inferred from the claim text.