Neokine protein and nucleic acid molecules and uses therefor
AssigneeMillennium Pharmaceuticals, Inc.
InventorsThomas M. Barnes, Charles Mackay
Life of this patent
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Abstract
Novel NEOKINE polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length NEOKINE proteins, the invention further provides isolated NEOKINE fusion proteins, antigenic peptides and anti-NEOKINE antibodies. The invention also provides NEOKINE nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals in which a NEOKINE gene has been introduced or disrupted. Diagnostic, screening and therapeutic methods utilizing compositions of the invention are also provided.
Claims22
An isolated nucleic acid molecule selected from the group consisting of:
- a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10 or SEQ ID NO:22, or a complement thereof;
- b) a nucleic acid molecule comprising a fragment of at least 100 contiguous nucleotides of a nucleic acid comprising the nucleotide sequence of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, or SEQ ID NO:22, or a complement thereof;
- c) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21;
- d) a nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21, wherein the fragment comprises at least 15 contiguous amino acid residues of the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21;
- and
- e) a nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21, wherein the nucleic acid molecule hybridizes to a nucleic acid molecule comprising SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, or SEQ ID NO:22 under stringent conditions.
An isolated polypeptide selected from the group consisting of:
- a) a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21;
- b) a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to a nucleic acid molecule comprising SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, or SEQ ID NO:22 under stringent conditions;
- c) a polypeptide which is encoded by the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, or SEQ ID NO:22;
- and
- d) a polypeptide comprising the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21.
A method for identifying a compound which modulates binding of NEOKINE to NEOKINE receptor comprising:
- a) contacting a cell which expresses NEOKINE receptor with NEOKINE and a test compound under conditions which allow NEOKINE to bind to NEOKINE receptor;
- and
- b) detecting binding of NEOKINE in the presence of the test compound;
- wherein a change in the amount of NEOKINE binding to NEOKINE receptor detected in the presence of the test compound indicates that the test compound modulates binding of NEOKINE to NEOKINE receptor.
A method for identifying a compound which modulates the activity of NEOKINE receptor comprising:
- a) contacting a cell which expresses NEOKINE receptor with NEOKINE and a test compound under conditions which allow NEOKINE to bind to NEOKINE receptor;
- and
- b) detecting the activity of NEOKINE receptor in the presence of the test compound;
- wherein a change in the activity NEOKINE receptor detected in the presence of the test compound indicates that the test compound modulates the activity of NEOKINE receptor.
A method for identifying a compound which modulates binding of NEOKINE to NEOKINE receptor comprising:
- a) contacting NEOKINE receptor with NEOKINE and a test compound under conditions which allow NEOKINE to bind to NEOKINE receptor;
- and
- b) detecting binding of NEOKINE in the presence of the test compound;
- wherein a change in the amount of NEOKINE binding to NEOKINE receptor detected in the presence of the test compound indicates that the test compound modulates binding of NEOKINE to NEOKINE receptor.
Dependencies are inferred from the claim text.