Method for labeling DNA and RNA
AssigneeU Chicago Argonne LLC
InventorsSergei Bavykin, Andrei D. Mirzabekov
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Abstract
A method for fragmenting and labeling nucleic acids is provided. The method comprises maintaining double- and single-stranded nucleic acid molecules in an aerobic or an anaerobic atmosphere, contacting the molecules with hydrogen peroxide and radical generating coordination complexes for a time and at concentrations sufficient to produce aldehyde moieties on the molecules, reacting the aldehyde moieties with amine to produce a condensation product, and labeling the condensation product.
Claims18
A method for labeling nucleic acids, the method comprising:
- a) contacting nucleic acid molecules with hydrogen peroxide and a redoxactive coordination complex for a time and at concentrations sufficient to produce free-aldehyde moieties on the molecules;
- b) reacting the aldehyde moieties with amine to produce a condensation product;
- and
- c) labeling the condensation product.
A method for modifying nucleic acids, the method comprising:
- a) contacting free radicals with the nucleic acids to produce free nucleic acid bases and aldehyde forms of ribose and deoxyribose;
- b) contacting the aldehyde forms with an amine to produce a condensation product;
- c) reducing the condensation product;
- and
- d) labeling the reduced condensation product.
Dependencies are inferred from the claim text.