Recombinant neospora antigens and their uses
AssigneeThe Regents of the University of California
InventorsPatricia C. Conrad, Bradd C. Barr, Mark L. Anderson, Karen W. Sverlow
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Abstract
The present invention provides isolated bovine Neospora cultures. Also provided are recombinant immunodominant Neospora antigens. The cultures and antigens are used to develop diagnostic assays for the detection of Neospora infections in cattle and other animals. Also provided are pharmaceutical compositions for the treatment and prevention of Neospora infections.
Claims27
An isolated recombinant nucleic acid construct comprising a nucleic acid encoding an immunodominant Neospora antigen.
A protein which has an amino acid sequence substantially identical to an amino acid sequence shown in SEQ ID NO:10.
A protein which has an amino acid sequence substantially identical to an amino acid sequence shown in SEQ ID NO:11.
A method of detecting the presence of antibodies specifically immunoreactive with a bovine Neospora antigen in a biological sample, the method comprising contacting the sample with an isolated recombinant immunodominant Neospora antigen, thereby forming an antigen/antibody complex, and detecting the presence or absence of the complex.
A method of detecting the presence of Neospora-specific nucleic acids in a biological sample, the method comprising contacting the sample with a oligonucleotide probe which specifically hybridizes with a target Neospora-specific polynucleotide sequence, thereby forming a hybridization complex, and detecting the presence or absence of the complex.
A pharmaceutical composition comprising a pharmaceutically acceptable carrier and an immunogenically effective amount of a bovine Neospora antigen.
A method for protecting a bovine animal from infection by bovine Neospora, the method comprising the administration of a pharmaceutical composition comprising a pharmaceutically acceptable carrier and an immunogenically effective amount of a bovine Neospora antigen.
Dependencies are inferred from the claim text.