Specific antibody directed to active hepatocyte growth factor activator and method for using the same
AssigneeMitsubishi Chemical Corporation
InventorsDaiji Naka, Kazuhiko Nagaike
Life of this patent
Estimating the term…
Abstract
An antibody which recognizes an active hepatocyte growth factor activator (HGFA) and does not substantially recognize inactive HGFA is provided. Also disclosed is a monoclonal antibody thereof, and a hybridoma cell line for producing the monoclonal antibody. There is further provided a method for measuring active HGFA using the antibody, and a method for detecting a disease, by detecting or measuring active HGFA using the antibody.
Claims30
An antibody that recognizes an active hepatocyte growth factor activator (HGFA) and does not substantially recognize inactive HGFA.
A monoclonal antibody that recognizes active HGFA activated by limited proteolysis of inactive HGFA, which is a precursor of active HGFA, between arginine at a position of 407 and isoleucine at a position of 408 counted from a translation initiation amino acid of inactive HGFA, and does not substantially recognize inactive HGFA.
A monoclonal antibody that recognizes active HGFA and does not substantially recognize inactive HGFA and a complex of active HGFA and a protease inhibitor.
A method for measuring active HGFA, comprising the step of measuring the active HGFA specifically by an immunological method using one or more kinds of antibodies that recognize an active hepatocyte growth factor activator (HGFA) and does not substantially recognize inactive HGFA.
A method for measuring active HGFA, comprising the step of measuring the active HGFA specifically by an immunological method using one or more kinds of antibodies that recognize active HGFA activated by limited proteolysis of inactive HGFA, which is a precursor of active HGFA, between arginine at a position of 407 and isoleucine at a position of 408 counted from a translation initiation amino acid of inactive HGFA, and does not substantially recognize inactive HGFA.
A method for measuring active HGFA, comprising the step of measuring the active HGFA specifically by an immunological method using one or more kinds of antibodies that recognize active HGFA and does not substantially recognize inactive HGFA and a complex of active HGFA and a protease inhibitor.
A method for detecting a disease, comprising the step of detecting or measuring active HGFA in a biological component collected from a subject suspected of having a disease.
A kit for detecting or measuring active HGFA, which comprises one or more kinds of antibodies that recognize an active hepatocyte growth factor activator (HGFA) and does not substantially recognize inactive HGFA.
A kit for detecting or measuring active HGFA, which comprises one or more kinds of antibodies that recognize an active HGFA activated by limited proteolysis of inactive HGFA, which is a precursor of active HGFA, between arginine at a position of 407 and isoleucine at a position of 408 counted from a translation initiation amino acid of inactive HGFA, and does not substantially recognize inactive HGFA.
A kit for detecting or measuring active HGFA, which comprises one or more kinds of antibodies that recognize active HGFA and does not substantially recognize inactive HGFA and a complex of active HGFA and a protease inhibitor.
A blood collection tube for collecting serum, plasma or whole blood, which is added with argatroban.
Dependencies are inferred from the claim text.