Methods for genetic immunization
OwnerHans Herweijer, Jon A. Wolff +3inventors
InventorsHans Herweijer, Jon A. Wolff, Aaron G. Loomis, Vladimir S. Trubetskoy, Mary Kay Bates
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Abstract
The present invention relates to methods for delivering a genetic immunogen, comprising a nucleic acid capable of expressing an antigen, optionally complexed with a polymer. The nucleic acid is delivered to the host via an intravascular, oral, buccal, dermal, nasal, or rectal route, where the complex transfects lymphoid tissues resulting in expression of an encoded antigen and induction of an antigen-specific immune response.
Claims33
A genetic immunization method for inducing an antigen-specific immune response, comprising:
- a nucleic acid sequence encoding a peptide containing at least one antigenic determinant, operatively linked to one or more control sequences such that the nucleic acid sequence is expressed in a host cell, wherein the nucleic acid sequence is optionally formulated into a particle by complexation with one or more polymers, and wherein the nucleic acid is delivered to a vertebrate host cell.
A genetic immunization composition formulated for inducing an antigen-specific immune response, comprising:
- a nucleic acid sequence encoding a peptide containing at least one antigenic determinant, operatively linked to one or more control sequences such that the nucleic acid sequence is expressible in a host cell, wherein the nucleic acid sequence is optionally formulated into a particle by complexation with a polymer, for delivery to a vertebrate host cell.
A method for generating an antibody response in a vertebrate host comprising of administering a nucleic acid encoding an antigen, the nucleic acid optionally being complexed to a polymer, in an amount sufficient to induce the desired immune response directed against the expressed antigen.
A method for generating a cellular immune response in a vertebrate host comprising of administering a nucleic acid encoding an antigen, the nucleic acid optionally being complexed to a polymer, in an amount sufficient to induce the desired immune response directed against the expressed antigen.
A method for generating an immune response in a vertebrate host, comprising:
- administering a nucleic acid encoding an antigen, the nucleic acid optionally being complexed to a polymer, in an amount sufficient to induce the desired immune response directed against the expressed antigen, and the nucleic acid is delivered to the intestinal lumen.
A method for determining the presence of a genetic immune response in a vertebrate, wherein the antigen is produced in a second vertebrate following nucleic acid delivery.
A method for determining the presence of a genetic immune response in a vertebrate, wherein the antigen is produced in a cell line following nucleic acid delivery.
A method for determining the presence of a genetic immune response in a vertebrate, wherein the antigen is produced in a primary cell culture following nucleic acid delivery.
A kit for genetic immunization, the kit comprising a transfection complex for in vivo gene transfer.
A kit for the detection of a genetic immune response, the kit comprising a transfection complex for in vitro gene transfer.
A kit for genetic immunization and detection of a genetic immune response, the kit comprising transfection complexes for in vivo and in vitro gene transfer.
Dependencies are inferred from the claim text.