Nucleic acid immunization
OwnerJoel R. Haynes, Michael D. Macklin +1inventors
InventorsJoel R. Haynes, Michael D. Macklin, Lendon G. Payne
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Abstract
Recombinant nucleic acid molecules are described. The molecules have a sequence or sequences encoding an influenza virus M2 antigen. Vectors and compositions containing these molecules are also described. Methods for eliciting an immune response using these molecules and compositions are also described.
Claims50
A polynucleotide vaccine composition comprising a nucleic acid sequence that encodes an influenza virus M2 antigen, wherein said nucleic acid sequence is not present in a recombinant viral vector.
A method for using an influenza virus M2 antigen to induce an immune response in a subject, said method comprising:
- (a) obtaining a nucleic acid sequence encoding the M2 antigen;
- (b) providing an expression cassette by linking the nucleic acid sequence to regulatory sequences such that the nucleic acid sequence is operatively linked to control sequences that direct expression of the M2 antigen when introduced into tissue of the subject, wherein said expression cassette is not present in a recombinant viral vector;
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- (c) administering the expression cassette to tissue of the subject.
A method of eliciting a protective immune response in a subject, said method comprising transfecting cells of the subject with a polynucleotide encoding an influenza virus M2 antigen, wherein said transfecting is carried out under conditions that permit expression of said antigen within the subject, said polynucleotide is not present in a recombinant viral vector, and said expression is sufficient to elicit a protective immune response against an influenza virus.
Dependencies are inferred from the claim text.