Methods and compositions for antisense VEGF oligonucleotides
OwnerParkash S. Gill, Rizwan Masoodinventors
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Abstract
This invention relates to compositions and methods for inhibition of abnormal proliferation of cells or angiogenesis. More particularly this invention provides VEGF antisense oligonucleotides capable of inhibiting proliferation of cancer cells or angiogenesis or combinations thereof. also provided are screening and prognostic assays, as well kits comprising the VEGF antisense oligonucleotides.
Claims18
A composition comprising an antisense oligonucleotides directed against vascular endothelial growth factor (VEGF), wherein said antisense oligonucleotide is UGGCTTGAAGATGTACTCGAU (SEQ ID NO:
- 34).
An antisense oligonucleotide having the sequence UGGCTTGAAGATGTACTCGAU (SEQ ID NO:
- 34).
A method for inhibiting cancer cell proliferation or angiogenesis comprising comprising contacting said cell with an antisense oligonucleotides directed against vascular endothelial growth factor (VEGF), wherein said antisense oligonucleotide is UGGCTTGAAGATGTACTCGAU (SEQ ID NO:
- 34).
A method of assessing the therapeutic potential of a candidate agent to inhibit cancer cell proliferation or angiogenesis, said method comprising:
- (i) contacting cells exhibiting autocrine growth activity with at least one candidate and (ii) measuring the level of VEGF expression or activity or cell growth, wherein an inhibition in VEGF expression or cell growth is indicative of the candidate agent's therapeutic potential.
A a prognostic assay for a subject afflicted with a disease involving abnormal cellular proliferation or angiogenesis, comprising::
- (i) isolating a biological sample a subject afflicted with a disease involving abnormal cellular proliferation (e.g., cancer) or angiogenesis;
- and (ii) evaluating said sample for autocrine VEGF activity or VEGF expression or VEGF receptor expression, wherein autocrine activity is indicative of a poorer prognosis for said
Dependencies are inferred from the claim text.