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US 2002/0165153 A1Published application
Transcription factors and their use
OwnerPeter Angel, Peter Herrlich +2inventors
InventorsPeter Angel, Peter Herrlich, Hans Van Dam, Alex Van Der Eb
Life of this patent
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Abstract
The invention relates to transcription factors with a DNA binding domain consisting of a basic domain and an adjacent leucine zipper domain. The aim of the invention is to provide modified transcription factors whose binding behavior towards each other and towards DNA sequences can be manipulated. To this end, at least one amino acid on the leucine zipper is replaced by a charged amino acid.
Claims5
A transcription factor with a DNA-binding domain comprising a basic domain and an adjacent Leucine zipper domain with the following amino acids:
- L el x gl x x x L e2 x g2 x x x′ x L e3 x g3 x x x L e4 x g4 x x x L e5
- wherein L is leucine, gi and ei are possible substitution locations, x are any amino acids, and x′ is one of tyrosin and glycin and wherein one of the following sets of substituents are at the nine substitution locations ei and gi:
- 2 KK KQ AT RQ K SEQ ID NO 3 EE EQ AT RQ K SEQ ID NO 4 EK KQ AT EE K SEQ ID NO 5 EE EQ AT EE K SEQ ID NO 6 KK EQ AT RQ K SEQ ID NO 7 KE EQ AT RQ K SEQ ID NO 8 KK KQ AT EE K SEQ ID NO 9 KE EQ AT EE K SEQ ID NO 10 EK KQ AT RQ E SEQ ID NO 11 KK KQ AT RQ E SEQ ID NO 12 EK EQ AT RQ E SEQ ID NO 13 EE EQ AT RQ E SEQ ID NO 14 EK KQ AT EE E SEQ ID NO 15 EE EQ AT EE E SEQ ID NO 16 KK SL QE RE K SEQ ID NO 18 EK SR QV RE K SEQ ID NO 19 KK SR QV RE K SEQ ID NO 20,
- valin being replaceable by alanine, leucine, methionine, isoleucine, glutamine.
1 more element
Dependencies are inferred from the claim text.