Brain-associated inhibitor of tissue-type plasminogen activator

An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 99% identical to a sequence selected from the group consisting of:

• (a) a nucleotide sequence encoding a polypeptide having the amino acid sequence consisting of residues n-410 of SEQ ID NO:
• 2, where n is an integer in the range of 2-49;
• (b) a nucleotide sequence encoding a polypeptide having the amino acid sequence consisting of residues 1-m of SEQ ID NO:
• 2, where n is an integer in the range of 381-409;
• (c) a nucleotide sequence encoding a polypeptide having the amino acid sequence consisting of residues n-m of SEQ ID NO:
• 2, where n is an integer in the range of 2-49 and m is an integer in the range of 381-409;
• (d) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete BAIT amino acid sequence encoded by the cDNA clone contained in ATCC Deposit 97722 wherein said portion excludes up to 48 amino acids from the amino terminus and up to 30 amino acids from the C-terminus of said complete amino acid sequence.

An isolated BAIT polypeptide having an amino acid sequence at least 97% identical to a sequence selected from the group consisting of:

• (a) the amino acid sequence of the BAIT polypeptide having the complete amino acid sequence in FIG. 1 (SEQ ID NO:
• 2);
• (b) the amino acid sequence of the mature BAIT polypeptide having the amino acid sequence at positions 19-410 in FIG. 1 (SEQ ID NO:
• 2);
• (c) the amino acid sequence of the BAIT polypeptide having the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97722;
• (d) the amino acid sequence of the mature BAIT polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97722;
• and
• (e) the amino acid sequence of an epitope-bearing portion of any one of the polypeptides of (a), (b), (c) or (d).

An isolated polypeptide comprising an epitope-bearing portion of the BAIT protein, wherein said portion is selected from the group consisting of:

• a polypeptide comprising amino acid residues from about Val 155 to about Ala 175 (SEQ ID NO:
• 2);
• a polypeptide comprising amino acid residues from about Phe 186 to about Pro 215 (SEQ ID NO:
• 2);
• a polypeptide comprising amino acid residues from about Tyr 225 to about Ile 239 (SEQ ID NO:
• 2);
• a polypeptide comprising amino acid residues from about Leu 243 to about Leu 255 (SEQ ID NO:
• 2);
• a polypeptide comprising amino acid residues from about Arg 380 to about Gly 386 (SEQ ID NO:
• 2);
• and a polypeptide comprising amino acid residues from about Met 395 to about Leu 410 (SEQ ID NO:
• 2).