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AssigneeUniversity of Maryland, College Park
InventorJ. Norman Hansen
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A sublancin peptide variant having a Gly-His peptide sequence fused to the C-terminal end of the mature sublancin peptide provides an affinity tag facilitating increased purification of the peptide variant from sample preparations without affecting the intracellular processing of the sublancin peptide variant, expression by a host cell or its biological activity in secreted form. This sublancin variant has specific inhibitory activity for spore outgrowth as for the native sublancin peptide. Production of the sublancin peptide variant on an industrial scale is set forth as are methods of decontaminating spore-infected areas. Methods for generating the peptide variant gene, plasmid and transformant are also described.
An affinity-tag labeled sublancin peptide comprising a chimeric polypeptide comprising a sublancin peptide, an amino acid spacer attached to the C-terminus of the sublancin peptide, and an affinity tag attached to the spacer.
A method for producing an affinity tag labeled sublancin peptide by a host cell comprising the steps of:
A method of purifying an affinity-tag labeled sublancin peptide from a solution, the peptide comprising a sublancin peptide, an amino acid spacer attached to the C-terminus of the sublancin peptide, and an affinity tag attached to the spacer, and wherein the method comprises contacting the peptide with a solid support having an affinity for the affinity tag.
A method for decontaminating a bacterial spore-infected area comprising treating the infected area with a spore-inhibiting effective amount of a peptide according to claim
Dependencies are inferred from the claim text.