US 2002/0165135 A1 · Compositions and methods related to canine IgG and canine IL-13 receptors

Abstract

The invention relates to canine immunoglobulin G (IgG) and canine interleukin-13 receptors (IL-13R) as well as fusion proteins containing canine IgG and/or canine IL-13R. In particular, the present invention discloses nucleic acid molecules encoding canine IgG, including species-specific regions of the heavy chain of canine IgG, and canine IL-13R alpha chain (IL-13Rα) proteins, particularly canine interleuken receptor alpha 1 (IL-13Rα1) and canine interleuken receptor alpha 2 (IL-13Rα2) proteins. Also included are canine IgG and IL-13Rα proteins, antibodies having selectivity for such proteins, inhibitors of such proteins and/or nucleic acid molecules, cells transformed with said nucleic acid molecules, assays employing such cells, nucleic acids molecules, proteins, antibodies and/or inhibitors, and therapeutic compositions comprising said nucleic acids molecules, proteins, antibodies and/or inhibitors. Also included are kits containing said molecules or chimera thereof, including their use to evaluate and regulate an immune response in an animal.

Claims59

Dependent claims

An isolated nucleic acid molecule, wherein said isolated nucleic acid molecule comprises a first nucleic acid sequence selected from the group consisting of:

(a) a second nucleic acid sequence which has at least 55% identity SEQ ID NO:1, SEQ ID NO:7, or SEQ ID NO:13, wherein said identity can be determined using a DNAsis computer program and default parameters;
(b) a third nucleic acid sequence which has at least 95% identity to SEQ ID NO:4, SEQ ID NO:
10, or SEQ ID NO:
16, wherein said identity is determined using the DNAsis computer program and default parameters;
(c) a fourth nucleic acid sequence which encodes a first amino acid sequence which has at least 40% identity to SEQ ID NO:2, or SEQ ID NO:
14, wherein said identity is determined using the DNAsis computer program and default parameters;
(d) a fifth nucleic acid sequence which encodes a second amino acid sequence which has at least 90% identity SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:
11 or SEQ ID NO:
17 wherein said identity is determined using the DNAsis computer program and default parameters;
(e) a sixth nucleic acid sequence which is an allelic variant of SEQ ID NO:
1, SEQ ID NO:4, SEQ ID NO:7, SEQ ID NO:
10, SEQ ID NO:
13, or SEQ ID NO:
16;
and
(f) a seventh nucleic acid sequence complementary to the second, third, fourth, fifth or sixth nucleic acid sequence.

13 more elements

An isolated nucleic acid molecule, wherein said isolated nucleic acid molecule comprises a first nucleic acid sequence selected from the group consisting of:

(a) a second nucleic acid sequence comprising at least 70 contiguous nucleotides of SEQ ID NO:1, SEQ ID NO:7 or SEQ ID NO:13;
(b) a third nucleic acid sequence comprising at least 350 contiguous nucleotides of SEQ ID NO:4, SEQ ID NO:10, and SEQ ID NO:
16;
(c) a fourth nucleic acid sequence comprising at least 450 contiguous nucleotides of SEQ ID NO:
19;
(d) a fifth nucleic acid sequence which encodes a first amino acid sequence comprising at least 20 contiguous residues of the sequence shown in SEQ ID NO:2, and SEQ ID NO:
14;
(e) a sixth nucleic acid sequence which encodes a second amino acid sequence comprising at least 100 contiguous residues of the sequence shown in SEQ ID NO:5,SEQ ID NO:8,SEQ ID NO:11, or SEQ ID NO:17;
(f) a seventh nucleic acid sequence which encodes a third amino acid sequence comprising at least 200 contiguous residues of the sequence shown in SEQ ID NO:20;
and
(h) an eighth nucleic acid sequence complementary to the second, third, fourth, fifth, sixth or seventh nucleic acid sequence.

8 more elements

An isolated nucleic acid molecule selected from the group consisting of:

(a) a first nucleic acid molecule comprising a first nucleic acid sequence encoding a first protein selected from the group consisting of:
(i) a second protein comprising an amino acid sequence that is at least 70 percent identical in sequence to SEQ ID NO:55, SEQ ID NO:58, SEQ ID NO:61, SEQ ID NO:66, and SEQ ID NO:69, wherein percent identity is determined by a DNAsis™ computer program with a gap penalty set at 5, the number of top diagonals set at 5, a fixed gap penalty set at 10, a k-tuple set at 2, a window size set at 10 and a floating gap penalty set at 10;
and
(ii) a second protein comprising a fragment of at least 40 contiguous amino acids identical in sequence to an at least 40 contiguous amino acids of the first protein;
(b) a second nucleic acid molecule comprising a second nucleic acid sequence encoding a protein that comprises an at least 30 contiguous amino acid region identical in sequence to an at least 30 contiguous amino acid region of SEQ ID NO:55, SEQ ID NO:58, SEQ ID NO:61, SEQ ID NO:66, and SEQ ID NO:69;
(c) a third isolated nucleic acid molecule complementary to the first or second nucleic acid molecule.

3 more elements

Dependencies are inferred from the claim text.

Compositions and methods related to canine IgG and canine IL-13 receptors

Life of this patent

Compositions and methods related to canine IgG and canine IL-13 receptors

Life of this patent

Abstract

Claims59