Method for inactivating gonadotrophs

A method for sterilizing a mammal, said method comprising administering an effective amount of a conjugate compound comprised of a peptide hormone selected from the group consisting of a GnRH analog (GnRH-A), a human chorionic gonadotropin, an equine chorionic gonadotropin, a luteinizing hormone and a follicle—stimulating hormone which is conjugated to a toxin selected from the group consisting of those plant toxins:

• ricin, modeccin, abrin, pokeweed anti-viral protein, a-amanitin, gelonin ribosome inhibiting protein (“RIP”) barley RIP, wheat RIP, corn RIP, rye RIP and flax RIP;
• those bacterial toxins:
• diphtheria toxin, pseudomonas exotoxin and shiga toxins having a toxic domain and a translocation domain or those chemical toxins:
• melphalan, methotrexate, nitrogen mustard, doxorubicin and daunomycin which are capable of direct chemical attack upon cells of said pituitary gland when conjugated to said peptide hormone.

A method for sterilizing a mammal, said method comprising administering an effective amount of a conjugate compound having the general formula:

• 4
• wherein X is an amino acid selected from the group consisting of lysine, D-lysine, ornithine, D-ornithine, glutamic acid, D-glutamic acid, aspartic acid, D-aspartic acid, cysteine, D-cysteine, tyrosine and D-tyrosine;
• Y is a linking agent selected from the group consisting of:
• 2-iminothiolane, N-succinimidyl-3-(2-pyridyldithio) propionate (SPDP), 4-succinimidyloxycarbonyl-α-(2-pyridyldithio)-toluene (SMPT), m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS), N-succinimidyl(4-iodoacetyl)aminobenzoate (SIAB), succinimidyl 4-(p-maleimidophenyl)butyrate (SMPB), 1-ethyl-3-(3-dimethylaminopropyl)carbodimide (EDC), bis-diazobenzidine or glutaraldehyde;
• Z is a substituent selected from the group consisting of Gly-NH2, ethylamide, and AzA-Gly-NH2 and T is a toxin selected from the group consisting of those plant toxins:
• ricin, modeccin, abrin, pokeweed anti-viral protein, a-amanitin, gelonin ribosome inhibiting protein (“RIP”) barley RIP, wheat RIP, corn RIP, rye RIP and flax RIP;
• those bacterial toxins:
• diphtheria toxin, pseudomonas exotoxin and shiga toxin having a toxic domain and a translocation domain or those chemical toxins:
• melphalan, methotrexate, nitrogen mustard, doxorubicin and daunomycin and wherein said conjugate compound is capable of binding with a receptor cell of the mammal's pituitary gland and direct attack upon cells of said pituitary gland.

A method for sterilizing a mammal, said method comprising administering an effective amount of a conjugate compound comprised of a peptide hormone selected from the group consisting of a GnRH analog (GnRH-A), a human chorionic gonadotropin, an equine chorionic gonadotropin, a luteinizing hormone and a follicle—stimulating hormone which is conjugated to a toxin selected from the group consisting of those plant toxins:

• ricin, modeccin, abrin, pokeweed anti-viral protein, a-amanitin, gelonin ribosome inhibiting protein (“RIP”) barley RIP, wheat RIP, corn RIP, rye RIP and flax RIP;
• those bacterial toxins:
• diphtheria toxin, pseudomonas exotoxin and shiga toxins having a toxic domain and a translocation domain or those chemical toxins:
• melphalan, methotrexate, nitrogen mustard, doxorubicin and daunomycin which are capable of direct chemical attack upon cells of said pituitary gland when conjugated to said peptide hormone.

A method for treating a sex hormone related disease in a mammal, said method comprising administering an effective amount of a conjugate compound having the general formula:

• 6
• wherein X is an amino acid selected from the group consisting of lysine, D-lysine, ornithine, D-ornithine, glutamic acid, D-glutamic acid, aspartic acid, D-aspartic acid, cysteine, D-cysteine, tyrosine and D-tyrosine;
• Y is a linking agent selected from the group consisting of:
• 2-iminothiolane, N-succinimidyl-3-(2-pyridyldithio) propionate (SPDP), 4-succinimidyloxycarbonyl-α-(2-pyridyldithio)-toluene (SMPT), m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS), N-succinimidyl(4-iodoacetyl)aminobenzoate (SIAB), succinimidyl 4-(p-maleimidophenyl)butyrate (SMPB), 1-ethyl-3-(3-dimethylaminopropyl)carbodimide (EDC), bis-diazobenzidine or glutaraldehyde;
• Z is a substituent selected from the group consisting of Gly-NH2, ethylamide, and AzA-Gly-N2 and T is a toxin selected from the group consisting of those plant toxins:
• ricin, modeccin, abrin, pokeweed anti-viral protein, a-amanitin, gelonin ribosome inhibiting protein (“RIP”) barley RIP, wheat RIP, corn RIP, rye RIP and flax RIP;
• those bacterial toxins:
• diphtheria toxin, pseudomonas exotoxin and shiga toxin having a toxic domain and a translocation domain or those chemical toxins:
• melphalan, methotrexate, nitrogen mustard, doxorubicin and daunomycin and wherein said conjugate compound is capable of binding with a receptor cell of the mammal's pituitary gland and direct attack upon cells of said pituitary gland.