Mass spectrometric immunoassay analysis of specific proteins and variants present in various biological fluids

A method for the isolation and subsequent qualitative characterization of target biomolecules present in biological fluid comprising the steps of:

• a. providing a MSIA-Tip having an affinity reagent present,
• b. separating and concentration the target biomolecule directly from the biological fluid by flowing a volume of the biological fluid through the MSIA-Tip, thereby binding the target biomolecules to the affinity reagent,
• c. cluting the target biomolecules onto a mass spectrometer target,
• d. performing mass spectrometric analysis on the target biomolecules in order to qualitatively determine the presence or absence of the target biomolecule in the biological fluid.

A method for the isolation and subsequent quantitative characterization of target biomolecules present in biological fluid comprising the steps of:

• a. adding a known amount of internal reference standard of predetermined concentration to a sample of the biological fluid,
• b. providing a MSIA-Tip having an affinity reagent present,
• c. flowing a volume of the biological fluid through the pipettor tip, thereby binding the target biomolecules to the affinity reagent,
• d. eluting the target biomolecules to a mass spectrometer target,
• e. performing mass spectrometric analysis on the target biomolecules in order to quantitatively determine the concentration of the target biomolecule in the biological fluid.