Immortal cell line derived from grouper Epinephelus coioides and its applications therein
AssigneeNational Science Council
InventorShau-Chi Chi
Life of this patent
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Abstract
The present invention describes (1) an immortal cell line derived from grouper and a method for establishing the cell line; (2) methods for mass producing and purifying aquatic 10 viruses using the immortal cell line from grouper; (3) an anti-NNV antibody and a method for producing the anti-NNV antibody; and (4) a vaccine of NNV and a method for protecting fish against NNV infection. The present immortal cell line is derived from the grouper and is susceptible to the viral families of Birnaviridae such as Infectious Pancreatic Necrosis Virus (IPNV); Herpesviridae such as Eel Herpes Virus Formosa (EHVF); Reoviridae such as Hard Clam Reovirus (HCRV); and Nodaviridae such as Nervous Necrosis Virus (NNV).
Claims36
An immortal cell line derived from grouper which is susceptible to aquatic viruses.
A method for establishing an immortal cell line comprising:
- establishing a primary cell culture by placing cells released from fin tissue of Epinephelus coioides in a tissue culture flask until a monolayer of cells is formed;
- subculturing and maintaining said monolayer of cells in a media suitable for cell subculturing;
- and
- monitoring the transformation of said subcultured cells which is characterized by susceptibility to aquatic viruses.
Dependencies are inferred from the claim text.