Multiple drug resistance gene atrD of aspergillus nidulans
AssigneeStichting voor de Technische Wetenschappen
InventorsPaul Luther Skatrud, Maarten A. de Waard, Alan C. Andrade, Robert Brown Peery
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Abstract
The invention provides isolated nucleic acid compounds encoding a multiple drug resistance protein of Aspergillus nidulans. Vectors and transformed host cells comprising the multiple drug resistance-encoding DNA of Aspergillus nidulans atrD are also provided. The invention further provides assays which utilize these transformed host cells.
Claims14
A DNA compound that comprises an isolated DNA sequence encoding SEQ ID NO:
- 2.
A method for determining the fungal MDR inhibition activity of a compound which comprises:
- a) placing a culture of fungal cells, transformed with a vector capable of expressing atrD, in the presence of:
- (i) an antifungal agent to which said fungal cell is resistant, but to which said fungal cell is sensitive in its untransformed state;
- (ii) a compound suspected of possessing Aspergillus nidulans MDR inhibition activity;
- and
- b) determining the fungal MDR inhibition activity of said compound by measuring the ability of the antifungal agent to inhibit the growth of said fungal cell.
The protein of SEQ ID No. 2 in purified form.
A strain of A. nidulans wherein said strain carries a gene disruption or gene replacement at the atrD locus such that said strain does not produce the atrD protein product.
Dependencies are inferred from the claim text.