Methods for lyophilizing competent cells
AssigneeInvitrogen Corporation
InventorsJoel A. Jessee, Fredric R. Bloom, Thuan Trinh
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Abstract
This invention relates to a method for producing cells which are competent for transformation and which may be stably stored for extended periods of time at various temperatures. The method involves growing cells in a growth conducive medium, rendering said cells competent, and lyophilizing said competent cells. The invention further relates to competent cells produced by such a method, to methods of transforming said cells with a DNA molecule, and to a method of producing a desired protein or polypeptide from said transformed cells.
Claims43
A method for producing cells which are competent for transformation, said method comprising
- (a) growing said cells in a growth conducive medium;
- (b) rendering said cells competent;
- and
- (c) lyophiling said competent cells.
Dependencies are inferred from the claim text.