Tissue plasminogen activator-like protease
AssigneeHuman Genome Sciences, Inc.
InventorsPaul A. Moore, Steven M. Ruben, Reinhard Ebner
Life of this patent
Estimating the term…
Abstract
The present invention relates to a novel t-PALP protein which is a member of the serine protease family. In particular, isolated nucleic acid molecules are provided encoding the human t-PALP protein. t-PALP polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of t-PALP activity. Also provided are diagnostic methods for detecting circulatory system-related disorders and therapeutic methods for treating circulatory system-related disorders.
Claims20
An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a member selected from the group consisting of:
- (a) a nucleotide sequence encoding the t-PALP polypeptide having the amino acid sequence at positions −21 to 242 of SEQ ID NO:2, or the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 209023;
- (b) a nucleotide sequence encoding the t-PALP polypeptide having the amino acid sequence at positions −20 to 242 of SEQ ID NO:2, or the complete amino acid sequence excepting the N-terminal methionine encoded by the cDNA clone contained in ATCC Deposit No. 209023;
- (c) a nucleotide sequence encoding the mature t-PALP polypeptide having the amino acid sequence at positions 1 to 242 of SEQ ID NO:2, or as encoded by the cDNA clone contained in ATCC Deposit No. 209023;
- (d) a nucleotide sequence encoding the kringle domain of the t-PALP polypeptide having the amino acid sequence at positions 4 to 63 of SEQ ID NO:2, or as encoded by the cDNA clone contained in ATCC Deposit No. 209023;
- (e) a nucleotide sequence encoding the protease domain of the t-PALP polypeptide having the amino acid sequence at positions 64 to 242 of SEQ ID NO:2, or as encoded by the cDNA clone contained in ATCC Deposit No. 209023;
- (f) a nucleotide sequence complementary to any of the nucleotide sequences in (a), (b), (c), (d) or (e) above.
An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a member selected from the group consisting of:
- (a) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of residues n to 242 of SEQ ID NO:2, where n is an integer in the range of −20 to 64;
- (b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of residues −20 to m of SEQ ID NO:2, where m is an integer in the range of 230 to 241;
- (c) a nucleotide sequence encoding a polypeptide having the amino acid sequence consisting of residues n to m of SEQ ID NO:2, where n and m are integers as defined respectively in (a) and (b) above;
- and
- (d) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete t-PALP amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 209023 wherein said portion excludes from 1 to about 63 amino acids from the amino terminus;
- (e) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete t-PALP amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 209023 wherein said portion excludes from 1 to about 11 amino acids from the carboxy terminus;
- and
- (f) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete t-PALP amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 209023 wherein said portion includes a combination of any of the amino terminal and carboxy terminal deletions in (d) and (e), above.
An isolated t-PALP polypeptide comprising an amino acid sequence at least 95% identical to a member selected from the group consisting of:
- (a) the amino acid sequence at positions −20 to 242 of SEQ ID NO:2, or the complete t-PALP amino acid sequence excepting the N-terminal methionine encoded by the cDNA clone contained in ATCC Deposit No. 209023;
- (b) the amino acid sequence of the mature form of the t-PALP polypeptide having the amino acid sequence at positions 1 to 242 of SEQ ID NO:2, or as encoded by the cDNA clone contained in the ATCC Deposit No. 209023;
- (c) the amino acid sequence of the kringle domain of the t-PALP polypeptide having the amino acid sequence at positions 4 to 63 of SEQ ID NO:2, or as encoded by the cDNA clone contained in the ATCC Deposit No. 209023;
- and
- (d) the amino acid sequence of the mature t-PALP polypeptide having the amino acid sequence at positions 64 to 242 of SEQ ID NO:2, or as encoded by the cDNA clone contained in the ATCC Deposit No. 209023;
- and
- (e) the amino acid sequence of an epitope-bearing portion t-PALP.
An isolated nucleic acid molecule comprising a polynucleotide having a sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) the nucleotide sequence of a portion of the sequence shown in FIG. 1 (SEQ ID NO:1) wherein said portion comprises at least 50 contiguous nucleotides from 1 to 110 or from 630 to 750;
- (b) the nucleotide sequence of a portion of the sequence shown in FIG. 1 (SEQ ID NO:1) wherein said portion consists of nucleotides 1 to 2000, 1 to 1500, 1 to 1000, 1 to 500, 1 to 250, 250 to 2000, 250 to 1500, 250 to 1000, 250 to 500, 500 to 2000, 500 to 1500, 500 to 1000, 1000 to 2000, or 1000 to 1500;
- and
- (c) a nucleotide sequence complementary to any of the nucleotide sequences in (a) or (b) above.
Dependencies are inferred from the claim text.