Dipeptidylpeptidases and methods of use
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsJames Travis, Jan S. Potempa, Agnieszka Banbula, Marcin Bugno
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Abstract
The present invention provides isolated polypeptides, dipeptidylpeptidases, active analogs, active fragments, or active modifications thereof, having amidolytic activity for cleavage of a peptide bond between the second and third amino acids from the N-terminal end of a target polypeptide, wherein the target polypeptide has an aliphatic or an aromatic residue as a substituent on the α-carbon atom of the second amino acid from the N-terminal end of the peptide. Isolated nucleic acids encoding dipeptidylpeptidases are also provided, as are methods of reducing growth of a bacterium by inhibiting a dipeptidylpeptidase.
Claims23
An isolated dipeptidylpeptidase, active analog, active fragment, or active modification thereof having amidolytic activity for cleavage of a peptide bond between the second and third amino acids from the N-terminal end of a target polypeptide, wherein the target polypeptide has an aliphatic or an aromatic residue as a substituent on the a-carbon atom of the second amino acid from the N-terminal end of the polypeptide.
An isolated polypeptide comprising an amino acid sequence having a percentage amino acid identity greater than about 40% with SEQ ID NO:2.
An isolated nucleic acid comprising a coding sequence encoding a dipeptidylpeptidase, active analog, active fragment, or active modification thereof having amidolytic activity for cleavage of a peptide bond between the second and third amino acids from the N-terminal end of a target polypeptide, wherein the target polypeptide has an aliphatic or an aromatic residue as a substituent on the α-carbon atom of the second amino acid from the N-terminal end of the polypeptide.
An isolated nucleic acid encoding a polypeptide, wherein the polypeptide comprises an amino acid sequence having a percentage amino acid identity greater than about 40% with SEQ ID NO:2.
A method of identifying an inhibitor of a dipeptidylpeptidase, active analog, active fragment, or active modification thereof, comprising identifying a compound that inhibits the amidolytic activity of the dipeptidylpeptidase by incubating the dipeptidylpeptidase with the compound under conditions that promote amidolytic activity of the dipeptidylpeptidase and determining if the amidolytic activity of the dipeptidylpeptidase is inhibited relative to the amidolytic activity in the absence of the compound.
A method of reducing growth of a bacterium comprising inhibiting a dipeptidylpeptidase, active analog, active fragment, or active modification thereof, by contacting the dipeptidylpeptidase with an inhibitor of the dipeptidylpeptidase.
A method for protecting an animal from a periodontal disease caused by Porphyromonas gingivalis comprising administering to the animal an inhibitor of dipeptidylpeptidase, wherein the disease is selected from the group consisting of gingivitis and periodontitis.
An immunogenic composition comprising an isolated dipeptidylpeptidase, an antigenic analog, an antigenic fragment, or an antigenic modification thereof having amidolytic activity for cleavage of a peptide bond present in a target polypeptide, the peptide bond being located between the second and third amino acids from the N-terminal end of the target polypeptide, wherein the second amino acid from the N-terminal end of the polypeptide has an aliphatic or an aromatic residue as a substituent on the a-carbon atom.
A composition comprising an inhibitor of an isolated dipeptidylpeptidase and a pharmaceutically acceptable carrier.
Dependencies are inferred from the claim text.