Complete nucleotide sequence of staphylococcus aureus ribosomal protein gene, S16 and methods for the indentification of antibacterial substances
OwnerJerry L. Slightom, John G. Chosay +2inventors
InventorsJerry L. Slightom, John G. Chosay, Dean L. Shinabarger, James D. Pearson
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Abstract
The invention provides an isolated S. aureus ribosomal polypeptide S16, and the isolated polynucleotide molecules that encode them, vectors and host cells comprising such polynucleotide molecules and also methods for the identification of agents that effect ribosomal assembly.
Claims48
An isolated nucleic acid comprising a nucleotide sequence that encodes an amino acid sequence having at least 85% identity with SEQ ID NO:2
An isolated nucleic acid comprising the nucleotide sequence having least 85% identity with SEQ ID NO:1
An isolated nucleic acid comprising a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:2
An isolated nucleic acid comprising the nucleotide sequence of SEQ ID NO:1
An isolated S16 ribosomal polypeptide comprising an amino acid sequence having least 85% identity to the sequence of SEQ ID NO:2
An isolated S16 ribosomal polypeptide comprising the amino acid sequence of SEQ ID NO:2
A method for testing for inhibitors of ribosomal assembly comprising the steps of:
- a.) contacting at least one direct binding ribosomal polypeptide selected from the group consisting of S4, S7, S8, S15, S17 and S20 with 16S ribosomal RNA to form a polyribonucleotide protein complex and;
- b) contacting said polyribonucleotide protein complex with S16 ribosomal polypeptide
- (i) in the presence of a test agent;
- and
- (ii) in the absence of said test agent;
- and
- c) determining the amount of S16 ribosomal polypeptide bound to the polyribonucleotide protein complex
- (i) in the presence of a test agent;
- and
- (ii) in the absence of said test agent;
- and
- d) comparing the amount of S16 ribosomal polypeptide determined in step (c)(i) to the amount of S16 ribosomal polypeptide determined in step (c)(ii);
A method for testing for inhibitors of ribosomal assembly comprising the steps of:
- a.) contacting at least one direct binding ribosomal polypeptide selected from the group consisting of S4, S7, S8, S15, S17 and S20 with 16S ribosomal RNA to form a polyribonucleotide protein complex and;
- b) contacting said polyribonucleotide protein complex with at least one non-direct binding ribosomal polypeptide selected from the group consisting of S1, S2, S3, S5, S6, S9, S10, S11, S12, S13, S14, S16, S18, S19, and S21.
- (iii) in the presence of a test agent;
- and
- (iv) in the absence of said test agent;
- and
- c) determining the amount of at least one non-direct binding ribosomal polypeptide bound to the RNA
- (i) in the presence of a test agent;
- and
- (ii) in the absence of said test agent;
- and
- d) comparing the amount of least one non direct binding ribosomal polypeptide in step (c)(i) to the amount of non-direct binding ribosomal polypeptide protein determined in step (c)(ii);
A method for testing for inhibitors of ribosomal assembly comprising the steps of:
- a.) contacting S4, S7, S8, S17 and S20 ribosomal polypeptides with 16S ribosomal RNA to form apolyribonucleotide protein complex and;
- b) contacting said polyribonucleotide protein complex with non-direct binding ribosomal polypeptides S3, S5, S9, S10, S12, S14, S16 and S19 to form a resultant polyribonucleotide protein complex
- (v) in the presence of a test agent;
- and
- (vi) in the absence of said test agent;
- and
- b) contacting non-direct binding ribosomal polypeptide S3 with said resultant polyribonucleotide protein complex;
- and
- c) determining the amount of said non-direct binding ribosomal polypeptide S3 bound to said resultant polyribonucleotide protein complex;
- (i) formed in the presence of said test agent;
- and
- (ii) formed in the absence of said test agent;
- and
- e) comparing the amount of S3 determined in step (c)(i) to the amount of S3 determined in step (c)(ii)
Dependencies are inferred from the claim text.