Polypeptides having acid phosphatase activity and nucleic acids encoding same
AssigneeNovozymes Biotech, Inc.
InventorsDebbie S. Yaver, Randy M. Berka, Michael W. Rey
Life of this patent
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Abstract
The present invention relates to isolated polypeptides having acid phosphatase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
Claims36
An isolated polypeptide having acid phosphatase activity, selected from the group consisting of:
- (a) a polypeptide having an amino acid sequence which has at least 65% identity with amino acids 19 to 318 for the mature polypeptide of SEQ ID NO. 2;
- (b) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under low stringency conditions with (i) nucleotides 138 to 1252 of SEQ ID NO. 1, (ii) the cDNA sequence contained in nucleotides 138 to 1252 of SEQ ID NO. 1, (iii) a subsequence of (i) or (ii) of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii), or (iii);
- (c) a variant of the polypeptide having an amino acid sequence of SEQ ID NO. 2 comprising a substitution, deletion, and/or insertion of one or more amino acids;
- (d) an allelic variant of (a) or (b);
- and
- (e) a fragment of (a), (b), or (d) that has acid phosphatase activity.
An isolated nucleic acid sequence comprising a nucleic acid sequence having at least one mutation in the mature polypeptide coding sequence of SEQ ID NO. 1, in which the mutant nucleic acid sequence encodes a polypeptide consisting of amino acids 19 to 318 of SEQ ID NO. 2.
An isolated nucleic acid sequence produced by (a) hybridizing a DNA under low stringency conditions with (i) nucleotides 138 to 1252 of SEQ ID NO. 1, (ii) the cDNA sequence contained in nucleotides 138 to 1252 of SEQ ID NO. 1, (iii) a subsequence of (i) or (ii) of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii), or (iii);
- and (b) isolating the nucleic acid sequence.
A method for producing a mutant nucleic acid sequence, comprising (a) introducing at least one mutation into the mature polypeptide coding sequence of SEQ ID NO. 1, wherein the mutant nucleic acid sequence encodes a polypeptide consisting of amino acids 19 to 318 of SEQ ID NO. 2;
- and (b) recovering the mutant nucleic acid sequence.
Dependencies are inferred from the claim text.