Soluble, active recombinant human MAO-B
OwnerMartin R. Deibel JR., Ronald D. Klein +1inventors
InventorsMartin R. Deibel JR., Ronald D. Klein, Anthony W. Yem
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Abstract
Monoamine oxidase B is an integral polypeptide of the outer mitochondrial membrane. Purified, soluble, biologically active human MAO-B is obtained by recombinant methods in amounts useful for high volume screening.
Claims17
A purified, soluble, biologically active human MAO-B polypeptide comprising the MAO-B amino acid sequence set forth in SEQ ID NO:
- 12.
A purified, soluble, biologically active human MAO-B polypeptide encoded by the nucleotide sequence selected from the group consisting of a) the MAO-B coding nucleotide sequence set forth in SEQ ID NO:
- 11, b) a nucleotide sequence that encodes the MAO-B amino acid sequence of SEQ ID NO:
- 12, and c) a nucleotide sequence that hybridizes to a) or b) under stringent conditions.
A method for producing a purified, soluble, biologically active human MAO-B polypeptide comprising:
- a) growing a host cell transformed with DNA encoding human MAO-B polypeptide under conditions permitting expression of said polypeptide;
- and
- e) isolating soluble, biologically active MAO-B polypeptide from said host cell or its medium of growth.
Dependencies are inferred from the claim text.