Cell calcification supressing proteins, and genes of the proteins
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsMasahiro Iwamoto, Yoshinobu Higuchi, Maurizio Pacifici, Joel Rosenbloom
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Abstract
This invention provides cell-calcification inhibitory proteins as well as genes encoding the proteins. Based on the discovery of a novel isoform gene of the c-erg gene (herein referred to as “C-11 gene”) which is an erg gene derived from chickens, the nucleotide sequence of the gene has been determined, and then the expression of a protein encoded by such gene (herein referred to as “C-11 protein”) has been confirmed. Further, it has been discovered that when the c-erg or C-11 gene is introduced into osteoblasts, the calcification of the cells is inhibited.
Claims19
A C-11 protein comprising a protein selected from the group consisting of:
- (a) a protein comprising an amino acid sequence having SEQ ID NO. 2 (SEQUENCE LISTING);
- and
- (b) a protein comprising an amino acid sequence that is derived from the amino acid sequence having SEQ ID NO. 2 by deletion, substitution or insertion of one or more amino acids, said protein having cell-calcification inhibitory activity.
A cell-calcification inhibitor comprising a c-erg protein selected from the group consisting of:
- (a) a protein comprising an amino acid sequence having SEQ ID NO. 4 (SEQUENCE LISTING);
- and
- (b) a protein comprising an amino acid sequence that is derived from the amino acid sequence having SEQ ID NO. 4 by deletion, substitution or insertion of one or more amino acids, said protein having cell-calcification inhibitory activity.
A method for measuring the calcification of cells comprising:
- measuring the expression of a C-11 gene or a c-erg gene in the cells.
A kit for measuring the cell-calcification of cells comprising either or both of an antibody to a C-11 protein and an antibody to a c-erg protein.
A method for screening a substance having cell-calcification inhibitory blocking activity, said method comprising using cells transformed with a gene encoding a protein selected from the group consisting of:
- (a) a protein comprising an amino acid sequence having SEQ ID NO. 2 (SEQUENCE LISTING);
- (b) a protein comprising an amino acid sequence that is derived from the amino acid sequence having SEQ ID NO. 2 by deletion, substitution or insertion of one or more amino acids, said protein having cell-calcification inhibitory activity;
- (c) a protein comprising an amino acid sequence having SEQ ID NO. 4. (SEQUENCE LISTING);
- and
- (d) a protein comprising an amino acid sequence that is derived from the amino acid sequence having SEQ ID NO. 4 by deletion, substitution or insertion of one or more amino acids, said protein having cell-calcification inhibitory activity.
A pharmaceutical composition comprising an erg protein.
A pharmaceutical composition comprising an erg gene.
A pharmaceutical composition comprising a C-11 protein or a c-erg protein.
A pharmaceutical composition comprising a C-11 gene or a c-erg gene.
A pharmaceutical composition comprising a protein having a consensus amino acid sequence between a c-erg protein and a C-11 protein.
Dependencies are inferred from the claim text.