Ginkgo biloba levopimaradiene synthase
AssigneeWilliam Marsh Rice University
InventorsSeiichi P.T. Matsuda, Hala G. Schepmann
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Abstract
The present invention is directed to nucleic acid sequences of Ginkgo biloba diterpene synthases, particularly of a levopimaradiene synthase. More specifically, the invention is directed to a cell of a unicellular organism, such as Saccharomyces cerevisiae or Escherichia coli, comprising levopimaradiene synthase for the metabolically engineered in vivo biosynthesis of a diterpene and a ginkgolide.
Claims67
A purified and isolated nucleic acid sequence encoding a levopimaradiene synthase.
A purified and isolated nucleic acid sequence comprising SEQ.ID.NO:1, SEQ.ID.NO:32, SEQ.ID.NO:34, SEQ.ID.NO:36 or SEQ.ID.NO:38.
A purified and isolated nucleic acid sequence comprising SEQ.ID.NO:34.
A purified and isolated nucleic acid sequence comprising SEQ.ID.NO:36.
An expression vector comprising an isolated and purified nucleic acid sequence encoding a levopimaradiene synthase under the control of a promoter operable in a host cell.
As a composition of matter, an isolated polypeptide comprising an amino acid sequence of a levopimaradiene synthase.
As a composition of matter, an isolated polypeptide comprising an amino acid sequence of SEQ.ID.NO:2, SEQ.ID.NO:33, SEQ.ID.NO:35, SEQ.ID.NO:37 or SEQ.ID.NO:39.
As a composition of matter, an isolated polypeptide comprising an amino acid sequence of SEQ.ID.NO:35.
As a composition of matter, an isolated polypeptide comprising an amino acid sequence of SEQ.ID.NO:37.
An expression vector comprising an isolated polynucleotide sequence encoding a polypeptide having an amino acid sequence of a levopimaradiene synthase.
An expression vector comprising an isolated polynucleotide sequence encoding a polypeptide having an amino acid sequence of SEQ.ID.NO:2, SEQ.ID.NO:33, SEQ.ID.NO:35, SEQ.ID.NO:37 or SEQ.ID.NO:39.
A unicellular organism comprising a purified and isolated nucleic acid sequence encoding a levopimaradiene synthase.
A unicellular organism comprising an isolated polynucleotide sequence encoding a polypeptide having an amino acid sequence of a levopimaradiene synthase.
A ginkgolide, wherein said ginkgolide is obtained from production in a unicellular organism, comprising a purified and isolated nucleic acid sequence encoding levopimaradiene synthase.
A ginkgolide, wherein said ginkgolide is obtained from production in a unicellular organism, comprising a purified and isolated nucleic acid sequence of SEQ.ID.NO:1, SEQ.ID.NO:32, SEQ.ID.NO:34, SEQ.ID.NO:36 or SEQ.ID.NO:38.
A ginkgolide, wherein said ginkgolide is obtained from production in a unicellular organism, wherein said organism comprises an expression vector comprising an isolated and purified nucleic acid sequence encoding a levopimaradiene synthase under the control of a promoter operable in said organism.
A ginkgolide, wherein said ginkgolide is obtained from production in a unicellular organism, wherein said organism comprises an isolated polynucleotide sequence encoding a polypeptide having an amino acid sequence of SEQ.ID.NO:2, SEQ.ID.NO:33, SEQ.ID.NO:35, SEQ.ID.NO:37 or SEQ.ID.NO:39.
As a composition of matter, a purified and isolated nucleic acid sequence comprising SEQ.ID.NO:5, SEQ.ID.NO:6, SEQ.ID.NO:7, SEQ.ID.NO:8, SEQ.ID.NO:9, SEQ.ID.NO:10, SEQ.ID.NO:11, SEQ.ID.NO:12, SEQ.ID.NO:29, SEQ.ID.NO:30, SEQ.ID.NO:31 or SEQ.ID.NO:40.
A transgenic plant, wherein said plant comprises an isolated and purified nucleic acid sequence encoding a levopimaradiene synthase under control of a promoter operable in said transgenic plant.
The plant of claim 63, wherein said nucleic acid sequence encoding said levopimaradiene synthase comprises SEQ.ID.NO:1, SEQ.ID.NO:32, SEQ.ID.NO:34, SEQ.ID.NO:36 or SEQ.ID.NO:38.
Dependencies are inferred from the claim text.