Procedure for the production of ethanol from lignocellulosic biomass using a new heat-tolerant yeast

Procedure for the production of ethanol from lignocellulosic biomass characterised in that it includes the stages of:

• Grinding the lignocellulosic biomass Subjecting the ground lignocellulosic biomass to steam explosion pre-treatment, maintaining at a pressure of between 1 and 3 MPa and a temperature between 190 and 230° C., for a period of time of between 1 and 10 minutes, depending on the type of material used and later provoking rapid de-pressurisation.
• Collecting the pre-treated material and separating the liquid and solid fractions by filtration, and introducing the solid fraction in the fermentation deposit (10).
• Adding a cellulase to the fermentation deposit (10) in a concentration of 15 UFP per gram of cellulose and 12.6 International Units of β-glucosidase enzyme. Inoculating the fermentation deposit (10) with a suspension of a culture of the heat-tolerant yeast Kluyveromyces marxianus CECT 10875. Shaking the mixture for 72 h at 42° C. Determining the concentration of ethanol and residual sugars in the mix, once the reaction is complete.