Method for reproducing in vitro the RNA-dependent RNA polymerase and terminal nucleotidyl transferase activities encoded by hepatitis C virus (HCV)
AssigneeIstituto di Ricerche di Biologia Molecolare P. Angeletti S.p.A.
InventorsRaffaele De Francesco, Licia Tomei, Sven-Erik Behrens
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Abstract
The present invention relates to the molecular biology and virology of the hepatitis C virus (HCV). An object of the present invention is a method to reproduce in vitro the RNA-dependent RNA polymerase activity of HCV that makes use of sequences contained in the HCV NS5B protein.
Claims7
A method for reproducing in vitro the RNA-dependent RNA polymerase activity or the terminal nucleotidyl transferase activity encoded by hepatitis C virus, characterized in that sequences containing NS5B (SEQ ID NO:
- 1) are used in the reaction mixture.
Method for reproducing in vitro the RNA-dependent RNA polymerase and terminal nucleotidyl transferase activities of NS5B, compositions of matter and use of said compositions of matter to set up an enzymatic test capable of selecting, for therapeutic purposes, compounds that inhibit the enzymatic activities associated with NS5B, according to the above description, examples and claims.
Dependencies are inferred from the claim text.