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US 2002/0164718 A1Published application
Metal ion affinity tags and methods for usig the same
AssigneeClontech Laboratories, Inc.
InventorsGrigoriy S. Tchaga, George G. Jokhadze
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Abstract
The present invention provides metal ion affinity peptides, fusion proteins comprising metal ion affinity peptides, and polynucleotides encoding the fusion proteins. The invention further provides recombinant vectors comprising subject polynucleotides, and host cells comprising the recombinant vectors. The invention further provides methods and kits for purifying a fusion protein comprising a metal ion affinity peptide.
Claims28
A metal ion affinity peptide of a formula selected from the group consisting of:
- formula 1:
- (His-X1-X2)n1-(His-X3-X4-X5)n2-(His-X6)n3, wherein each of X1 and X2 is independently an amino acid with an aliphatic or an amide side chain, each of X3, X4, X5 is independently an amino acid with a basic side chain (except His) or an acidic side chain, each X6 is an amino acid with an aliphatic or an amide side chain, n1 and n2 are each independently 1-3, and n3 is 1-5;
- formula 2:
- (His-Asn)n, where n=3 to 10;
- and
- formula 3:
- (His-X1-X2)n, wherein each of X1 and X2 is an amino acid having an acidic side chain, and n=3 to 10.
4 more elements
Dependencies are inferred from the claim text.