Platelet-derived growth factor D, DNA coding therefor, and uses thereof
AssigneesLudwig Institute for Cancer Research, Licentia Ltd.
InventorsUlf Eriksson, Karin Aase, Xuri Li, Annica Ponten, Marko Uutela, Kari Alitalo, Arne Oestman, Carl-Henrik Heldin
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Abstract
PDGF-D, a new member of the PDGF/VEGF family of polypeptide growth factors, is described, as well as nucleotide sequences encoding, methods for producing, pharmaceutical compositions containing this new growth factor, and its antibodies and other antagonists. Also disclosed are transfected and transformed host cells expressing PDGF-D, and uses thereof in medical and diagnostic applications.
Claims29
An isolated nucleic acid molecule comprising a polynucleotide encoding a polypeptide having a PDGF-D activity and having a sequence identity of at least 85% with at least nucleotides 1 to 600 of SEQ ID NO:3, at least nucleotides 1 to 966 of SEQ ID NO:5, at least nucleotides 176-1288 SEQ ID NO:7, at least nucleotides 938 to 1288 of SEQ ID NO:7, at least nuleotides 1-1110 of SEQ ID NO:35, or at least nucleotides 1-1092 of SEQ ID NO:37, or a polynucleotide which hybridizes under stringent conditions with at least one of said sequences.
An isolated polypeptide having a biological activity of PDGF-D and comprising an amino acid sequence having at least 85% identity with SEQ ID NOs:4, 6, 8, 36, 38, or at least the amino acid residues 255 to 371 of SEQ ID NO:8, or a fragment or analog thereof having the biological activity of PDGF-D.
An isolated nucleic acid molecule which codes for a polypeptide comprising a characteristic sequence of SEQ ID NO:25.
A means for amplifying a nucleic acid molecule encoding a polypeptide comprising a sequence selected from the group consisting of SEQ ID NOs:
- 4, 6, 8, 36 and 38, said means comprising at least one primer specific for the sequence.
A method of identifying specific types of human tumors, comprising the steps of taking a sample of said tumor, testing said sample for the expression of PDGF-D, and identifying tumor type based on PDGF-D expression test result.
An isolated nucleic acid molecule which codes for a polypeptide comprising the sequence of SEQ ID NO:36.
An isolated nucleic acid molecule which codes for the polypeptide comprising the sequence of SEQ ID NO:38.
An isolated nucleic acid molecule which codes for the polypeptide comprising the sequence of SEQ ID NO:40.
Dependencies are inferred from the claim text.