High level promoters from cyanobacteria
OwnerLisa L. Huang, Robert A. Larossa +1inventors
InventorsLisa L. Huang, Robert A. Larossa, Michael P. McCluskey
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Abstract
The invention relates to the field of microbiology. More specifically, methods are provided for the identification of highly expressed genes and their corresponding promoters and UV responsive genes and their corresponding promoters in cyanobacteria Synechocystis sp. PCC6803. These genes and promoters can be used to construct expression vectors in cyanobacteria, green algae or plants, for the production of biomaterials from sunlight, a renewable energy resource.
Claims16
A method for regulating expression of a coding region of interest in a cyanobacterium comprising:
- a) providing a transformed cyanobacterium having a gene fusion comprising:
- i) a promoter region from a gene selected from the group consisting of:
- 1) an amiC gene or an rbcX gene;
- and
- 2) a gene having a nucleotide sequence as set forth in SEQ ID NO:
- 5;
- and
- ii) a coding region of interest;
- wherein the promoter region is operably linked to the coding region of interest;
- and
- b) culturing the transformed cyanobacterium of step (a), in the log phase whereby the promoter region is activated and the coding region of interest is expressed.
A method for regulating expression of a coding region of interest in a cyanobacterium comprising:
- a) providing a transformed cyanobacterium having a gene fusion comprising:
- i) a promoter region from a gene selected from the group consisting of:
- 1) an hliB gene, an hsp17 gene, a nblB gene, a rpoD gene, an hliA gene, a ftsH gene and a clpB gene;
- and
- 2) a gene having a nucleotide sequence selected from the group consisting of SEQ ID NOs:9, 11, 17, 21, 25, 27, 31, and 39;
- and
- ii) a coding region of interest;
- wherein the promoter region is operably linked to the coding region of interest;
- and
- b) culturing the transformed cyanobacterium of step (a) in the presence of UV-B light, whereby the promoter region is activated and the coding region of interest is expressed.
Dependencies are inferred from the claim text.