Card-domain containing polypeptides, encoding nucleic acids, and methods of use
OwnerKrzysztof Pawlowski, John C. Reed +1inventors
InventorsKrzysztof Pawlowski, John C. Reed, Adam Godzik
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Abstract
The invention provides caspase recruitment domain (CARD)-containing polypeptides and functional fragments thereof, encoding nucleic acid molecules, and specific antibodies. Also provided are screening methods for identifying CARD-associated polypeptides (CAPs), and for identifying agents that alter the association of a CARD-containing polypeptide with itself or with a CAP. Further provided are methods of altering a biochemical process modulated by a CARD-containing polypeptide, and methods of diagnosing a pathology characterized by an increased or decreased level of a CARD-containing polypeptide.
Claims28
An isolated nucleic acid molecule encoding a CARD-containing polypeptide, wherein said nucleic acid molecule is selected from the group consisting of:
- (a) a nucleic acid molecule encoding a polypeptide comprising the amino acid sequence of CARD-11X (SEQ ID NO:8) or CARD-12X (SEQ ID NO:16);
- (b) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:7 or SEQ ID NO:15;
- and
- (c) a nucleic acid molecule that hybridizes to the nucleic acid molecule of (a) or (b) under moderately stringent hybridization conditions.
An isolated nucleic acid molecule encoding a functional fragment of a CARD-containing polypeptide, wherein said nucleic acid molecule is selected from the group consisting of:
- (a) a nucleic acid molecule encoding the CARD domain of CARD-10X (SEQ ID NO:4), the filament domain of CARD-10X (SEQ ID NO:6), the CARD domain of CARD11X (SEQ ID NO:10), the ERM (ezrin) domain of CARD-11X (SEQ ID NO:12), the PDZ domain of CARD-11X (SEQ ID NO:14) and the CARD domain of CARD-12X (SEQ ID NO:16);
- (b) a nucleic acid molecule comprising the nucleotide sequence of the CARD domain of CARD-10X (SEQ ID NO:3), the filament domain of CARD-10X (SEQ ID NO:5), the CARD domain of CARD-11X (SEQ ID NO:9), the ERM (ezrin) domain of CARD-11X (SEQ ID NO:11), the PDZ domain of CARD-11X (SEQ ID NO:13) and the CARD domain of CARD-12X (SEQ ID NO:15);
- and
- (c) a nucleic acid molecule that hybridizes to the nucleic acid molecule of (a) or (b) under moderately stringent hybridization conditions,
- wherein said nucleic acid molecule does not consist of the nucleotide sequence set forth as SEQ ID NOS:19 or 21-37.
A nucleic acid molecule comprising substantially the same nucleotide sequence as SEQ ID NO:7 or SEQ ID NO:15.
An oligonucleotide comprising at least 15 contiguous nucleotides of the nucleotide sequence set forth in any of SEQ ID NOS:1, 7 or 15, or the complement thereof, wherein said nucleic acid molecule does not consist of the nucleotide sequence set forth as SEQ ID NOS:19 or 21-37.
A substantially purified CARD-containing polypeptide, comprising substantially the same amino acid sequence as the amino acid sequence of CARD-11X (SEQ ID NO:8) or CARD-12X (SEQ ID NO:16).
A substantially purified functional fragment of a CARD-containing polypeptide, comprising substantially the same amino acid sequence as the amino acid sequence of the CARD domain of CARD-10X (SEQ ID NO:4), the filament domain of CARD-10X (SEQ ID NO:6), the CARD domain of CARD-11X (SEQ ID NO:10), the ERM (ezrin) domain of CARD-11X (SEQ ID NO:12), the PDZ domain of CARD-11X (SEQ ID NO:14) and the CARD domain of CARD-12X (SEQ ID NO:16),
- wherein said functional fragment is not encoded by a nucleic acid molecule consisting of the nucleotide sequence set forth as SEQ ID NOS:19 or 21-37.
A substantially purified functional fragment of a CARD-containing polypeptide, comprising at least 10 contiguous residues of SEQ ID NOS:
- 2, 8 or 16,
- wherein said functional fragment is not encoded by a nucleic acid molecule consisting of the nucleotide sequence set forth as SEQ ID NOS:19 or 21-37, and
- wherein said functional fragment is immunogenic.
Dependencies are inferred from the claim text.