Metabolic rate shifts in fermentations expressing recombinant proteins
AssigneeGenentech, Inc.
InventorsDana Andersen, John Joly, Bradley R. Snedecor
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Abstract
The invention provides a method for increasing product yield of a polypeptide of interest produced by recombinant host cells, where expression of the polypeptide by the recombinant host cells is regulated by an inducible system. More specifically, the method involves culturing the recombinant host cells under conditions of high metabolic and growth rate and then reducing the metabolic rate of the recombinant host cells at the time of induction of polypeptide expression. In particular, the invention provides a method of increasing product yield of an antibody, growth factor, or protease produced by a recombinant E. coli host cell regulated by an inducible system
Claims23
A method for increasing product yield of a polypeptide of interest produced by recombinant host cells, wherein expression of the polypeptide by the recombinant host cells is regulated by an inducible system, which method comprises
- (a) culturing the recombinant host cells under conditions of high metabolic and growth rate;
- and
- (b) reducing metabolic rate of the recombinant host cells at the time of induction of polypeptide expression.
A method of increasing product yield of an antibody, growth factor, or protease produced by a recombinant E. coli host cell, wherein expression of the antibody, growth factor, or protease is regulated by an inducible system, which method comprises
- (a) culturing the host cells under conditions of high metabolic and growth rate;
- and
- (b) reducing metabolic rate of the recombinant host cells at the time of induction of antibody, growth factor, or protease expression.
Dependencies are inferred from the claim text.