Mammalian cerberus-like protein and compositions
OwnerMaximillian Follettie, Edward M. Derobertisinventors
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Abstract
Purified mammalian cerberus proteins and processes for producing them are disclosed. DNA molecules encoding the mammalian cerberus proteins are also disclosed. The proteins may be used for inducing formation, growth, differentiation, proliferation and/or maintenance of neurons and/or related neural cells and tissues such as Schwann cells, glial cells and astrocytes, and for other tissue repair, including cardiac and endoderm.
Claims25
An isolated DNA sequence comprising a DNA sequence selected from the group consisting of:
- (a) nucleotides from nucleotides beginning at # 1, 52, 55, 58, 61, 64, 67, 70, 73, 121, 256, 259, 262, 265, 268, 271 or 484 and ending at #723 or 801 of SEQ ID NO:7;
- and
- (b) sequences which hybridize to (a) under stringent hybridization conditions and encode a protein which exhibits cerberus activity.
An isolated DNA sequence comprising a DNA sequence selected from the group consisting of:
- (a) nucleotides encoding amino acids starting with amino acids beginning at # 1, 18 to 25, 41, 85 to 91 or 162 and ending at #241 or 267 of SEQ ID NO:
- 8;
- and
- (b) sequences which hybridize to (a) under stringent hybridization conditions and encode a protein which exhibits cerberus activity.
An isolated DNA molecule comprising a DNA sequence selected from the group consisting of:
- (a) nucleotide #268 to #801 of SEQ ID NO:2;
- and
- (b) naturally occurring allelic sequences and equivalent degenerative codon sequences of (a).
An isolated DNA molecule encoding mammalian cerberus protein, said DNA molecule comprising nucleotide #268 to #801 of SEQ ID NO:7.
A purified mammalian cerberus polypeptide comprising an amino acid sequence according to SEQ ID NO:8.
A purified mammalian cerberus protein produced by the steps of
- (a) culturing a cell transformed with a DNA comprising the nucleotide sequence from nucleotide #268 to #801 as shown in SEQ ID NO:7;
- and
- (b) recovering and purifying from said culture medium a protein comprising the amino acid sequence from amino acid #90 to amino acid #267 as shown in SEQ ID NO:8.
A purified mammalian cerberus protein comprising the amino acid sequence from amino acid #1 to #267 of SEQ ID NO:8.
A purified mammalian cerberus protein comprising the amino acid sequence from amino acid #90 to #267 of SEQ ID NO:2.
A purified mammalian cerberus protein produced by the steps of
- (a) culturing a cell transformed with a DNA comprising the nucleotide sequence from nucleotide #52 to #801 as shown in SEQ ID NO:7;
- and
- (b) recovering and purifying from said culture medium a protein comprising the amino acid sequence from amino acid #18 to amino acid #267 as shown in SEQ ID NO:8.
Dependencies are inferred from the claim text.