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US 2002/0164677 A1Published application
High-throughput microbial culturing
AssigneeThe State of Oregon Acting by and through the State Board of Higher Education on Behlalf of
InventorsStephen Giovannoni, Stephanie Connon
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Abstract
Methods for isolating microbial species, particularly previously uncultured species, from non-laboratory source environments (such as soil, freshwater, seawater, etc.) are disclosed. These methods can include the use of cultures in arrays, and cells deposited on solid surfaces in arrays for detection, and flow cytometry and/or cell sorting and/or dilution cultures.
Claims26
A method of isolating a microbial species from a source environment, comprising:
- gathering at least one microorganism from the source environment;
- providing a volume of culture medium to the microorganism in at least one microtiter plate compartment;
- incubating the microorganism in the medium for a period of time and in an environment sufficient to result in growth of the microorganism if the medium and environment are capable of supporting such growth;
- and
- detecting growth of the microorganism.
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Dependencies are inferred from the claim text.