Method and apparatus for performing multiple processing steps on a sample in a single vessel
AssigneeIrm LLC
InventorRobert Charles Downs
Life of this patent
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Abstract
A method is provided for determining crystallization conditions for a protein, the method comprising: performing in a same fermentation apparatus 2 or more separate fermentations where each fermentation comprises cells expressing a protein to be purified and has a fermentation volume of less than 500 mL; purifying the expressed protein from the separate fermentations; and determining crystallization conditions for the purified protein by submicroliter crystallization experiments.
Claims81
A method comprising:
- performing a plurality of fermentations, each fermentation in a different sample vessel;
- performing a further processing step on the plurality of fermented samples where the sample is retained in the same sample vessel as the fermentation during the processing step;
- isolating recombinant proteins expressed during the fermentations after the further processing step;
- and,
- crystallizing the isolated recombinant proteins.
A system for processing a plurality of samples comprising:
- a first station for performing a plurality of fermentations, each fermentation being performed in a different sample vessel;
- a second station for performing a further processing step on the plurality of fermented samples where the sample is retained in the same sample vessel as the fermentation during the further processing step;
- and,
- means for crystallizing proteins expressed in the samples.
A method for producing a purified protein, the method comprising:
- performing in a same fermentation apparatus 2 or more separate fermentations where each fermentation comprises cells expressing a protein to be purified and has a fermentation volume of less than 500 mL;
- and
- purifying the expressed protein from the separate fermentations.
A method for determining crystallization conditions for a protein, the method comprising:
- performing in a same fermentation apparatus 2 or more separate fermentations where each fermentation comprises cells expressing a protein to be purified and has a fermentation volume of less than 500 mL;
- purifying the expressed protein from the separate fermentations;
- and
- determining crystallization conditions for the purified protein by submicroliter crystallization experiments.
Dependencies are inferred from the claim text.