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OwnerJurg Gysin, Artur Scherfinventors
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Conformational antigens and antibodies recognizing said antigens, a process for the efficient generation of monoclonal antibodies to native or conformational antigens expressed or carried by eukaryotic cells. A process for the selection of conformational antigens, use of monoclonal antibodies for therapeutical, diagnostic or vaccine applications. The process for preparing monoclonal antibodies, comprises: rendering an animal tolerant to an eukaryotic cell in a first state; detecting said tolerant animal; immunizing said tolerant animal, by injection of the eukaryotic cell in a second state carrying a neo-antigen or a non-self antigen; fusing B cells of said immunized mice with a myeloma cell line; and selecting the hybridoma expressing antibodies against said neo-antigen or non-self antigen.
A process for preparing monoclonal antibodies, comprising:
A process for selecting a native or conformational antigen, comprising:
The hybridoma Pf 26G1/B4 deposited at Collection Nationale de Cultures de Microorganismes (CNCM) on Feb. 23, 2001, under accession number 1-2635.
The hybridoma Pf 26G1/C10 deposited at Collection Nationale de Cultures de Microorganismes (CNCM) on Feb. 23, 2001, under accession number I-2636.
A hybridoma which secretes an antibody having the same epitope specificity as the antibody produced by hybridoma Pf 26G1/B4 deposited at Collection Nationale de Cultures de Microorganismes (CNCM) on Feb. 23, 2001, under accession number I-2635.
A hybridoma which secretes an antibody having the same epitope specificity as the antibody produced by hybridoma Pf 26G1/C10 deposited at Collection Nationale de Cultures de Microorganismes (CNCM) on Feb. 23, 2001, under accession number I-2636.
A conformational antigen selected and characterized by its capacity to react with monoclonal antibody obtained by a process which is 200 uptimes greater successful than classical process to obtain similar hybridoma.
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