MyD88 adapter-like proteins
AssigneeImmunex Corporation
InventorsJohn E. Sims, Timothy A. Bird, Luke A.J. O'Neill
Life of this patent
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Abstract
There are disclosed MAL-1 polypeptides and active fragments of MAL-1, including fragments that associate with IRAK2. Further disclosed are DNAs encoding the polypeptides and fragments as well as methods of using the DNAs and polypeptides.
Claims18
A polynucleotide that encodes a fragment of the polypeptide of SEQ ID NO:2, wherein the fragment is capable of associating with IRAK2 or the fragment is capable of associating with MyD88.
A polynucleotide encoding a polypeptide selected from the group consisting of:
- a) amino acids 68-236 of SEQ ID NO:2;
- b) amino acids 86-236 of SEQ ID NO:2;
- c) amino acids 86-217 of SEQ ID NO:2 and,
- d) amino acids 68-217 of SEQ ID NO:2.
- e) amino acids x-236 of SEQ ID NO:2 where x is any of amino acids 68-86 of SEQ ID NO:2;
- f) amino acid x1-217 of SEQ ID NO:2, where x1 is any of amino acids 68-86 of SEQ ID NO:2;
- wherein the polypeptide of a) through f) is capable of associating with IRAK2.
A polynucleotide that encodes the polypeptide of SEQ ID NO:2 with the proviso that the Pro at amino acid 125 is His.
A method for screening a test compound to identify its ability to affect the association of a polypeptide with IRAK2, the method comprising the steps of:
- a. forming a composition comprising IRAK2, the polypeptide, and the test compound;
- and
- b. assaying for the level of interaction of the polypeptide and IRAK2 such that if the level obtained in (b) differs from that obtained in the absence of test compound, a compound that affects the interaction of the polypeptide and IRAK2 is identified;
- and, wherein, the polypeptide comprises the polypeptide of SEQ ID NO:2 or fragment of the polypeptide of SEQ ID NO:2 and the fragment is capable of associating with IRAK2.
A method for screening a test compound to identify its ability to affect the association of a polypeptide with MyD88, the method comprising the steps of:
- a) forming a composition comprising MyD88, the polypeptide, and the test compound;
- and
- b) assaying for the level of interaction of the polypeptide and MyD88, such that if the level obtained in (b) differs from that obtained in the absence of test compound, a compound that affects the interaction of the polypeptide and MyD88s identified;
- and, wherein, the polypeptide comprises the polypeptide of SEQ ID NO:2 or a fragment of the polypeptide of SEQ ID NO:2 and the fragment is capable of associating with MyD88.
Dependencies are inferred from the claim text.