Process for determining antibiotics containing a b-lactam ring in biological fluid

Abstract

The present invention relates to novel processes for detecting antibiotics containing a β-lactam ring in a biological fluid, comprising the following steps a) placing a determined volume of the said biological fluid in contact with an amount of recognition agent and incubating the mixture thus obtained under conditions which allow the complexation of the antibiotics, which may be present in the said biological fluid, with the recognition agent, b) placing the mixture obtained in step a) in contact with at least one reference antibiotic immobilized on a support, under conditions which allow the complexation of the reference antibiotic with the amount of recognition agent which has not reacted in step a), and c) determining the amount of recognition agent bound to the support, characterized in that the recognition agent comprises a receptor which is sensitive to the antibiotics containing a β-lactam ring obtained from Bacillus licheniformis.

Dependencies are inferred from the claim text.