Methods for stool sample preparation
OwnerAnthony P. Shuber, Stanley N. Lapidus +1inventors
InventorsAnthony P. Shuber, Stanley N. Lapidus, Gail E. Radcliffe
Life of this patent
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Abstract
The present invention provides methods for the preparation of stool samples to increase the yield of relevant DNA, and further provides methods for isolating and analyzing target DNA for characteristics indicative of colorectal cancer. Methods for screening patients for the presence of cancerous or pre-cancerous colorectal lesions are also provided.
Claims40
A method for analyzing DNA extracted from stool, comprising:
- homogenizing a stool sample in a solvent for DNA in order to form a homogenized sample mixture having a solvent volume to stool mass ratio of at least 5:1;
- enriching said homogenized sample for human DNA;
- and
- analyzing said human DNA for characteristics of disease.
A method of screening for the presence of a colorectal cancerous or pre-cancerous lesion in a patient, the method comprising the steps of:
- obtaining a sample comprising at least a cross-sectional portion of a stool voided by the patient;
- homogenizing the sample in a solvent in order to form a homogenized sample mixture having a solvent volume to stool mass ratio of at least 5:1;
- enriching said sample for a target human DNA;
- and
- analyzing the target human DNA for DNA characteristics indicative of the presence of said colorectal cancerous or pre-cancerous lesion.
A method for screening for the presence of a colorectal cancerous or precancerous lesion in a patient, the method comprising the steps of:
- obtaining a sample comprising at least a cross-sectional portion of a stool voided by the patient;
- homogenizing the sample in a solvent in order to form a homogenized sample mixture having a solvent volume to stool mass ratio of at least 5:1;
- ensuring that said sample have at least a minimum number N of total DNA molecules to provide for detection of a low-frequency target DNA molecule;
- analyzing the target DNA for DNA characteristics indicative of the presence of said colorectal cancerous or pre-cancerous lesion.
A method for screening for the presence of a colorectal cancerous or precancerous lesion in a patient, the method comprising the steps of:
- obtaining a sample containing of least a cross-sectional portion of a stool voided by the patient;
- homogenizing the sample in a solvent in order to form a homogenized sample mixture having a solvent volume to stool mass ratio of at least 5:1;
- enriching said homogenized sample for target human DNA;
- ensuring that said sample have at least a minimum number N of total DNA molecules to provide for detection of a low-frequency target DNA molecule;
- analyzing the target human DNA for DNA characteristics indicative of the presence of said colorectal cancerous or pre-cancerous lesion.
Dependencies are inferred from the claim text.