Compositions isolated from bovine tissues and methods for their use
AssigneeGenesis Research & Development Corporation Limited
InventorsMatthew Glenn, Murray R. Grigor, Adrian J. Molenaar, Stephen R. Davis
Life of this patent
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Abstract
Isolated polynucleotides encoding histatin polypeptides expressed in bovine tissues, including mammary gland and paratoid salivary gland tissues, are provided, together with genetic constructs and host cells comprising such isolated polynucleotides. Methods for the use of such polynucleotides and polypeptides are also provided.
Claims16
An isolated polynucleotide comprising a sequence selected from the group consisting of:
- (1) sequences recited in SEQ ID NOS:
- 1-6;
- (2) complements of the sequences recited in SEQ ID NOS:
- 1-6;
- (3) reverse complements of the sequences recited in SEQ ID NOS:
- 1-6;
- (4) reverse sequences of the sequences recited in SEQ ID NOS:
- 1-6;
- (5) sequences having at least a 99% probability of being the same as a sequence recited in (1)-(4) above as determined using computer algorithm BLASTN;
- (6) sequences having at least 75% identity to a nucleotide sequence recited in (1)-(4) above determined using computer algorithm BLASTN;
- (7) sequences having at least 90% identity to a nucleotide sequence recited in (1)-(4) above determined using computer algorithm BLASTN;
- (8) sequences having at least 95% identity to a nucleotide sequence recited in (1)-(4) above determined using computer algorithm BLASTN;
- (9) nucleotide sequences that hybridize to a sequence recited in (1)-(4) above under stringent hybridization conditions;
- (10) nucleotide sequences that are 200-mers of a sequence recited in (1)-(4) above;
- (11) nucleotide sequences that are 100-mers of a sequence recited in (1)-(4) above;
- (12) nucleotide sequences that are 40-mers of a sequence recited in (1)-(4) above;
- (13) nucleotide sequences that are 20-mers of a sequence recited in (1)-(4) above;
- and (14) nucleotide sequences that are degeneratively equivalent to a sequence recited in (1)-(4) above.
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
- (a) sequences provided in SEQ ID NOS:
- 7-14;
- (b) sequences having at least a 99% probability of being the same as a sequence of SEQ ID NOS:
- 7-14, as determined using the computer algorithm BLASTP;
- (c) sequences having at least 75% identity to a sequence provided in SEQ ID NOS:
- 7-14, as determined using the computer algorithm BLASTP;
- (d) sequences having at least 90% identity to a sequence provided in SEQ ID NOS:
- 7-14, as determined using the computer algorithm BLASTP;
- and (e) sequences having at least 95% identity to a sequence provided in SEQ ID NOS:
- 7-14, as determined using the computer algorithm BLASTP.
An isolated polypeptide comprising at least a functional portion of an amino acid sequence selected from the group consisting of:
- (a) sequences provided in SEQ ID NOS:
- 7-12;
- (b) sequences having at least a 99% probability of being the same as a sequence of SEQ ID NOS:
- 7-12, as determined using the computer algorithm BLASTP;
- (c) sequences having at least 75% identity to a sequence provided in SEQ ID NOS:
- 7-12, as determined using the computer algorithm BLASTP;
- (d) sequences having at least 90% identity to a sequence provided in SEQ ID NOS:
- 7-12, as determined using the computer algorithm BLASTP;
- and (e) sequences having at least 95% identity to a sequence provided in SEQ ID NOS:
- 7-12, as determined using the computer algorithm BLASTP.
Dependencies are inferred from the claim text.