Enzymes having high temperature polymerase activity and methods of use thereof
AssigneeDiversa Corporation
InventorsWalter Callen, Eric J. Mathur
Life of this patent
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Abstract
The invention relates to thermostable polymerases that have polymerase activity temperatures in the range from 90° C. up to 113° C., such as those derived from Pyrolobus fumaria, and to polynucleotides encoding the polymerases In addition, methods of designing new thermostable DNA polymerases and methods of use thereof are also provided. The polymerases have increased activity and stability at increased pH and temperature.
Claims8
A computer readable medium having stored thereon a nucleic acid sequence as set forth in SEQ ID NO:
- 1, and sequences substantially identical thereto, or a polypeptide sequence as set forth in SEQ ID NO:
- 2, and sequences substantially identical thereto.
A computer system comprising a processor and a data storage device wherein said data storage device has stored thereon a nucleic acid sequence as set forth in SEQ ID NO:
- 1, and sequences substantially identical thereto, or a polypeptide sequence as set forth in SEQ ID NO:
- 2, and sequences substantially identical thereto.
A method for comparing a first sequence to a reference sequence wherein said first sequence is a nucleic acid sequence as set forth in SEQ ID Nos:
- 1, and sequences substantially identical thereto, or a polypeptide sequence as set forth in SEQ ID NO:
- 2, and sequences substantially identical thereto comprising:
- reading the first sequence and the reference sequence through use of a computer program which compares sequences;
- and
- determining differences between the first sequence and the reference sequence with the computer program.
A method for identifying a feature in a sequence wherein the sequence is as set forth in SEQ ID NO:
- 1, sequences substantially identical thereto, or a polypeptide sequence of SEQ ID NO:
- 2, and sequences substantially identical thereto comprising:
- reading the sequence through the use of a computer program which identifies features in sequences;
- and
- identifying features in the sequences with the computer program.
Dependencies are inferred from the claim text.