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US 2002/0164614 A1Published application
Method and kit for enhancing the association rates of polynucleotides
AssigneeGen-Probe Incorporated
InventorMichael M. Becker
Life of this patent
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Abstract
The present invention features a method and associated kit for increasing the association rate between polynucleotides having complementary, single-stranded regions, where the method includes providing to a test sample containing the complementary polynucleotides one or more synthetic, water soluble polycationic polymers.
Claims60
A method for forming a duplex from a polynucleotide probe and a target nucleic acid, said method comprising the steps of:
- providing said probe to a test sample under conditions permitting said probe to preferentially hybridize to said target nucleic acid, if present, in said sample;
- and
- providing a synthetic polycationic polymer to said sample in an amount sufficient to increase the association rate of said probe and said target nucleic acid in said sample under said conditions.
A kit comprising:
- a polynucleotide probe which preferentially hybridizes to a target nucleic acid present in a test sample under a first set of hybridization conditions;
- and
- a synthetic polycationic polymer in an amount sufficient to increase the association rate of said probe and said target nucleic acid in said sample under said first set of hybridization conditions.
Dependencies are inferred from the claim text.