Oligonucleotide identifiers
OwnerR. Shoshana Bamdad, Cynthia C. Bamdadinventors
Life of this patent
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Abstract
Methods, assays, and components are described in which biological samples can be rapidly and sensitively analyzed for the presence of species associated with neurodegenerative disease. Techniques and components are provided for diagnosis of disease, as well as for screening of candidate drugs for treatment of neurodegenerative disease. The techniques are simple, extremely sensitive, and utilize readily-available components. Binding species, capable of binding a neurodegenerative disease aggregate-forming or aggregate-forming species, are fastened to surfaces of electrodes and surfaces of particles, or provided free in solution, to bind aggregate-forming species and/or be involved in aggregation.
Claims118
A method comprising:
- allowing a chemical or biological species, immobilized relative to a surface, to participate in a chemical or biological interaction;
- and
- determining participation of the chemical or biological species in the chemical or biological interaction by identifying an oligonucleotide identifier associated with the surface.
A kit comprising:
- an article having a surface;
- a chemical or biological species, able to participate in a chemical or biological interaction, fastened to or adapted to be fastened to the surface;
- and
- an oligonucleotide identifier fastened to or adapted to be fastened to the surface.
A kit comprising a plurality of particles each carrying a chemical or biological functionality allowing it to fasten to a binding partner, and each carrying an identical oligonucleotide linker constructed for attachment to a complementary oligonucleotide fastened to an oligonucleotide identifier.
A composition comprising:
- a chemical or biological species, able to participate in a chemical or biological interaction;
- a linker species that is not a ribosome;
- and
- an oligonucleotide identifier, wherein each of the chemical or biological species and the oligonucleotide identifier is fastened to or adapted to be fastened to the linker species.
A composition comprising:
- a protein;
- an linker species that is not a ribosome;
- and
- an oligonucleotide identifier that encodes for the protein, wherein each of the protein and the oligonucleotide identifier is immobilized relative to, or adapted to be immobilized relative to, the linker species.
A kit comprising:
- a surface;
- a protein immobilized relative to the surface or adapted to be immobilized relative to the surface;
- and
- an oligonucleotide identifier that codes for the protein, immobilized relative to the surface or adapted to be immobilized relative to the surface.
A kit comprising:
- a polymer or dendrimer;
- a protein immobilized relative to the polymer or dendrimer or adapted to be immobilized relative to the polymer or dendrimer;
- and
- an oligonucleotide identifier that codes for the protein, immobilized relative to the polymer or dendrimer or adapted to be immobilized relative to the polymer or dendrimer.
A composition comprising:
- a protein and an oligonucleotide identifier that codes for the protein, immobilized relative to each other or adapted to be immobilized relative to each other.
A kit comprising:
- a protein and an oligonucleotide identifier that codes for the protein, immobilized relative to each other or adapted to be immobilized relative to each other;
- and
- an entity carrying immobilized thereto a binding partner of the protein.
A method comprising:
- expressing a protein with an oligonucleotide;
- immobilizing the protein and the oligonucleotide relative to each other.
A method comprising:
- allowing a chemical or biological species to participate in a chemical or biological interaction;
- and
- determining participation of the chemical or biological species in the chemical or biological interaction by identifying an oligonucleotide identifier, wherein the oligonucleotide identifier encodes the chemical or biological species.
Generating a library of nucleic acids that contain components of a cDNA library and a functionality to facilitate binding to a surface.
Generating a library of nucleic acids that contain components of a cDNA library and a functionality the products of which are used in an in vitro assay.
Generating a library of nucleic acids that contain components of a cDNA library and sequences to which nucleic acid binding proteins bind.
Generating a library of plasmids that contain components of a cDNA library and a functionality to facilitate binding to a surface.
Generating a library of plasmids that contain components of a cDNA library and a functionality the products of which are used in an in vitro assay.
Generating a library of plasmids that contain components of a cDNA library and sequences to which nucleic acid binding proteins bind.
Generating a library of nucleic acids or plasmids that contain components of a cDNA library, sequences that encode a DNA binding domain and sequences to which the encoded DNA binding domain binds.
Generating a library of nucleic acids or plasmids that contain components of a cDNA library, sequences that encode a DNA binding domain and sequences to which the encoded DNA binding domain binds, wherein the binding motif sequences are not in proximity to a reporter gene.
A kit comprising:
- at least one colloid particle;
- at least one magnetic bead;
- at least one protein recognition motif adapted for immobilization to the at least one colloid particle;
- and
- an uncharacterized protein or drug adapted for immobilization to the at least one bead.
A method comprising:
- exposing a plurality of colloid particles, each carrying an immobilized protein recognition motif, to a bead carrying an immobilized, uncharacterized protein or drug;
- and
- determining immobilization of at least one particle to the bead via interaction between the protein recognition motif and the uncharacterized protein or drug.
Dependencies are inferred from the claim text.