Method for typing a cell
OwnerCarol G. Leggett, Ellyn Whitehouse +1inventors
InventorsCarol G. Leggett, Ellyn Whitehouse, Robert H. Reeves
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Abstract
A method for typing a cell includes the steps of: (a) providing a cell of unknown type, the cell of unknown type having a nucleic acid forming a portion of the cell's genome being IGR1, IGR2, ETS1, or ETS2; (b) isolating the nucleic acid from the cell of unknown type; (c) amplifying the nucleic acid by PCR to form a PCR product; (d) digesting the PCR product with a restriction endonuclease to form a plurality of restriction fragments; (e) separating the plurality of restriction fragments to generate a restriction pattern; and (f) comparing the restriction pattern generated in step (e) with a restriction pattern of a cell of known type.
Claims10
A method for typing a cell, the method comprising the steps of:
- (a) providing a cell of unknown type, the cell of unknown type having a nucleic acid forming a portion of the cell's genome selected from the group consisting of IGR1, IGR2, ETS1, and ETS2;
- (b) isolating the nucleic acid from the cell of unknown type;
- (c) amplifying the nucleic acid by PCR to form a PCR product;
- (d) digesting the PCR product with a restriction endonuclease to form a plurality of restriction fragments;
- (e) separating the plurality of restriction fragments to generate a restriction pattern;
- and
- (f) comparing the restriction pattern generated in step (e) with a restriction pattern of a cell of known type.
Dependencies are inferred from the claim text.